Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector

Varsani, A., Williamson, A. L., Stewart, D., & Rybicki, E. P. (2006) Transient expression of Human papillomavirus type 16 L1 protein in Nicotiana benthamiana using an infectious tobamovirus vector. Virus Research, 120(1-2), pp. 91-96.

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Abstract

A Tobacco mosaic virus (TMV)-derived vector was used to express a native Human papillomavirus type 16 (HPV-16) L1 gene in Nicotiana benthamiana by means of infectious in vitro RNA transcripts inoculated onto N. benthamiana plants. HPV-16 L1 protein expression was quantitated by enzyme-linked immunosorbent assays (ELISA) after concentration of the plant extract. We estimated that the L1 product yield was 20-37 μg/kg of fresh leaf material. The L1 protein in the concentrated extract was antigenically characterised using the neutralising and conformation-specific Mabs H16:V5 and H16:E70, which bound to the plant-produced protein. Particles observed by transmission electron microscopy were mainly capsomers but virus-like particles (VLPs) similar to those produced in other systems were also present. Immunisation of rabbits with the concentrated plant extract induced a weak immune response. This is the first report of the successful expression of an HPV L1 gene in plants using a plant virus vector. © 2006 Elsevier B.V. All rights reserved.

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ID Code: 54933
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996): 28
Export Date: 12 November 2012
Source: Scopus
Keywords: HPV-16, Papillomavirus, Plant expression, TMV vector, Vaccine, VLP
DOI: 10.1016/j.virusres.2006.01.022
Deposited On: 20 Nov 2012 02:46
Last Modified: 20 Nov 2012 02:57

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