A prime-boost immunisation regimen using recombinant BCG and Pr55 gag virus-like particle vaccines based on HIV type 1 subtype C successfully elicits Gag-specific responses in baboons
Chege, G. K., Thomas, R., Shephard, E. G., Meyers, A., Bourn, W., Williamson, C., Maclean, J., Gray, C. M., Rybicki, E. P., & Williamson, A. L. (2009) A prime-boost immunisation regimen using recombinant BCG and Pr55 gag virus-like particle vaccines based on HIV type 1 subtype C successfully elicits Gag-specific responses in baboons. Vaccine, 27(35), pp. 4857-4866.
Mycobacterium bovis BCG is considered an attractive live bacterial vaccine vector. In this study, we investigated the immune response of baboons to a primary vaccination with recombinant BCG (rBCG) constructs expressing the gag gene from a South African HIV-1 subtype C isolate, and a boost with HIV-1 subtype C Pr55 gag virus-like particles (Gag VLPs). Using an interferon enzyme-linked immunospot assay, we show that although these rBCG induced only a weak or an undetectable HIV-1 Gag-specific response on their own, they efficiently primed for a Gag VLP boost, which strengthened and broadened the immune responses. These responses were predominantly CD8+ T cell-mediated and recognised similar epitopes as those targeted by humans with early HIV-1 subtype C infection. In addition, a Gag-specific humoral response was elicited. These data support the development of HIV-1 vaccines based on rBCG and Pr55 gag VLPs. © 2009 Elsevier Ltd. All rights reserved.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996): 12 Export Date: 12 November 2012 Source: Scopus|
|Keywords:||Baboons, HIV vaccines, Recombinant BCG, Virus-like particles|
|Deposited On:||20 Nov 2012 02:46|
|Last Modified:||29 May 2013 16:54|
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