Inhibition of maize streak virus (MSV) replication by transient and transgenic expression of MSV replication-associated protein mutants
Shepherd, D. N., Mangwende, T., Martin, D. P., Bezuidenhout, M., Thomson, J. A., & Rybicki, E. P. (2007) Inhibition of maize streak virus (MSV) replication by transient and transgenic expression of MSV replication-associated protein mutants. Journal of General Virology, 88(1), pp. 325-336.
Maize streak disease is a severe agricultural problem in Africa and the development of maize genotypes resistant to the causal agent, Maize streak virus (MSV), is a priority. A transgenic approach to engineering MSV-resistant maize was developed and tested in this study. A pathogen-derived resistance strategy was adopted by using targeted deletions and nucleotide-substitution mutants of the multifunctional MSV replication-associated protein gene (rep). Various rep gene constructs were tested for their efficacy in limiting replication of wild-type MSV by co-bombardment of maize suspension cells together with an infectious genomic clone of MSV and assaying replicative forms of DNA by quantitative PCR. Digitaria sanguinalis, an MSV-sensitive grass species used as a model monocot, was then transformed with constructs that had inhibited virus replication in the transient-expression system. Challenge experiments using leafhopper-transmitted MSV indicated significant MSV resistance - from highly resistant to immune - in regenerated transgenic D. sanguinalis lines. Whereas regenerated lines containing a mutated full-length rep gene displayed developmental and growth defects, those containing a truncated rep gene both were fertile and displayed no growth defects, making the truncated gene a suitable candidate for the development of transgenic MSV-resistant maize. © 2007 SGM.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996): 11 Export Date: 12 November 2012 Source: Scopus|
|Deposited On:||20 Nov 2012 02:46|
|Last Modified:||20 Nov 2012 02:57|
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