Replicative intermediates of maize streak virus found during leaf development

Erdmann, J. B., Shepherd, D. N., Martin, D. P., Varsani, A., Rybicki, E. P., & Jeske, H. (2010) Replicative intermediates of maize streak virus found during leaf development. Journal of General Virology, 91(4), pp. 1077-1081.

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Abstract

Geminiviruses of the genera Begomovirus and Curtovirus utilize three replication modes: complementary-strand replication (CSR), rolling-circle replication (RCR) and recombinationdependent replication (RDR). Using two-dimensional gel electrophoresis, we now show for the first time that maize streak virus (MSV), the type member of the most divergent geminivirus genus, Mastrevirus, does the same. Although mastreviruses have fewer regulatory genes than other geminiviruses and uniquely express their replication-associated protein (Rep) from a spliced transcript, the replicative intermediates of CSR, RCR and RDR could be detected unequivocally within infected maize tissues. All replicative intermediates accumulated early and, to varying degrees, were already present in the shoot apex and leaves at different maturation stages. Relative to other replicative intermediates, those associated with RCR increased in prevalence during leaf maturation. Interestingly, in addition to RCR-associated DNA forms seen in other geminiviruses, MSV also apparently uses dimeric open circular DNA as a template for RCR. © 2010 SGM.

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ID Code: 54968
Item Type: Journal Article
Refereed: Yes
Additional Information: Cited By (since 1996): 9
Export Date: 12 November 2012
Source: Scopus
DOI: 10.1099/vir.0.017574-0
Deposited On: 20 Nov 2012 02:46
Last Modified: 29 May 2013 16:54

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