Restoration of native folding of single-stranded DNA sequences through reverse mutations: an indication of a new epigenetic mechanism
Shepherd, D. N., Martin, D. P., Varsani, A., Thomson, J. A., Rybicki, E. P., & Klump, H. H. (2006) Restoration of native folding of single-stranded DNA sequences through reverse mutations: an indication of a new epigenetic mechanism. Archives of Biochemistry and Biophysics, 453(1), pp. 108-122.
We used in vivo (biological), in silico (computational structure prediction), and in vitro (model sequence folding) analyses of single-stranded DNA sequences to show that nucleic acid folding conservation is the selective principle behind a high-frequency single-nucleotide reversion observed in a three-nucleotide mutated motif of the Maize streak virus replication associated protein (Rep) gene. In silico and in vitro studies showed that the three-nucleotide mutation adversely affected Rep nucleic acid folding, and that the single-nucleotide reversion [C(601)A] restored wild-type-like folding. In vivo support came from infecting maize with mutant viruses: those with Rep genes containing nucleotide changes predicted to restore a wild-type-like fold [A(601)/G(601)] preferentially accumulated over those predicted to fold differently [C(601)/T(601)], which frequently reverted to A(601) and displaced the original population. We propose that the selection of native nucleic acid folding is an epigenetic effect, which might have broad implications in the evolution of plants and their viruses.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996): 6
Export Date: 12 November 2012
|Deposited On:||20 Nov 2012 02:46|
|Last Modified:||29 May 2013 16:54|
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