An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins
Windram, O. P., Weber, B., Jaffer, M. A., Rybicki, E. P., Shepherd, D. N., & Varsani, A. (2008) An investigation into the use of human papillomavirus type 16 virus-like particles as a delivery vector system for foreign proteins: N- and C-terminal fusion of GFP to the L1 and L2 capsid proteins. Archives of Virology, 153(3), pp. 585-589.
Development of vaccine strategies against human papillomavirus (HPV), which causes cervical cancer, is a priority. We investigated the use of virus-like particles (VLPs) of the most prevalent type, HPV-16, as carriers of foreign proteins. Green fluorescent protein (GFP) was fused to the N or C terminus of both L1 and L2, with L2 chimeras being co-expressed with native L1. Purified chimaeric VLPs were comparable in size (∼55 nm) to native HPV VLPs. Conformation-specific monoclonal antibodies (Mabs) bound to the VLPs, thereby indicating that they possibly retain their antigenicity. In addition, all of the VLPs encapsidated DNA in the range of 6-8 kb. © 2007 Springer-Verlag.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996): 5 Export Date: 12 November 2012 Source: Scopus|
|Deposited On:||20 Nov 2012 02:46|
|Last Modified:||29 May 2013 16:54|
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