HIV-1 sub-type C chimaeric VLPs boost cellular immune responses in mice
Pillay, S., Shephard, E. G., Meyers, A. E., Williamson, A. L., & Rybicki, E. P. (2010) HIV-1 sub-type C chimaeric VLPs boost cellular immune responses in mice. Journal of Immune Based Therapies and Vaccines, 8.
Several approaches have been explored to eradicate HIV; however, a multigene vaccine appears to be the best option, given their proven potential to elicit broad, effective responses in animal models. The Pr55 Gagprotein is an excellent vaccine candidate in its own right, given that it can assemble into large, enveloped, virus-like particles (VLPs) which are highly immunogenic, and can moreover be used as a scaffold for the presentation of other large non-structural HIV antigens. In this study, we evaluated the potential of two novel chimaeric HIV-1 Pr55 Gag-based VLP constructs - C-terminal fusions with reverse transcriptase and a Tat::Nef fusion protein, designated GagRT and GagTN respectively - to enhance a cellular response in mice when used as boost components in two types of heterologous prime-boost vaccine strategies. A vaccine regimen consisting of a DNA prime and chimaeric HIV-1 VLP boosts in mice induced strong, broad cellular immune responses at an optimum dose of 100 ng VLPs. The enhanced cellular responses induced by the DNA prime-VLP boost were two- to three-fold greater than two DNA vaccinations. Moreover, a mixture of GagRT and GagTN VLPs also boosted antigen-specific CD8+ and CD4+ T-cell responses, while VLP vaccinations only induced predominantly robust Gag CD4+ T-cell responses. The results demonstrate the promising potential of these chimaeric VLPs as vaccine candidates against HIV-1. © 2010 Pillay et al; licensee BioMed Central Ltd.
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|Item Type:||Journal Article|
|Additional Information:||Cited By (since 1996): 2
Export Date: 12 November 2012
Art. No.: 7
|Deposited On:||20 Nov 2012 02:46|
|Last Modified:||20 Nov 2012 02:57|
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