Effect of a novel compound from Lycopodium obscurum L. on osteogenic activity of osteoblasts in vitro

Wang, Chaoyuan, Wu, Ruiyun, Yang, Guangzhong, Blackwood, Keith A., Friis, Thor, Hutmacher, Dietmar W., & Woodruff, Maria A. (2013) Effect of a novel compound from Lycopodium obscurum L. on osteogenic activity of osteoblasts in vitro. Natural Science, 5(1), pp. 84-92.

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Abstract

We investigated the potential of an extract of Lycopodium obscurum L.; stigmastane-3-oxo-21-oic acid (SA), to enhance osteogensis of mouse osteoblastic MC3T3-E1 cells. SA at a concentration of 16 µM was found to have no significant effect upon the viability of the cells, thus concentrations of 8 µM and 16 µM of SA were used in all further experiments. Both concentrations of SA had an inhibitory affect upon alkaline phosphatase activity (ALP) after 8 days incubation, however, after 16 days activity was restored to control levels. However Alizarin red S staining showed increased levels of mineralization for both concentrations after 16 days culture. Real time PCR showed inhibition of genes Runx2 and Osterix genes responsible for the up-regulation of ALP. However early time point (8 days) up-regulation of bone matrix mineralization genes OPN and OCN, and late time point (16 days) up-regulation of both Jun-D and Fra-2 mRNA expression was significantly enhanced. These results suggest a potential me-chanism of SA in enhancing bone fracture healing is through the up-regulating bone matrix minera-lization.

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ID Code: 55475
Item Type: Journal Article
Refereed: Yes
DOI: 10.4236/ns.2013.51014
ISSN: 2150-4105 (online) 2150-4091 (print)
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)
Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300)
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 SciRes.
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Deposited On: 11 Dec 2012 23:12
Last Modified: 11 May 2013 15:31

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