Contribution of DEAF1 structural domains to the interaction with the breast cancer oncogene LMO4

Cubeddu, Liza, Joseph, Soumya, Richard, Derek J., & Matthews, Jacqueline M. (2012) Contribution of DEAF1 structural domains to the interaction with the breast cancer oncogene LMO4. PLoS ONE, 7(6), e39218.

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Abstract

The proteins LMO4 and DEAF1 contribute to the proliferation of mammary epithelial cells. During breast cancer LMO4 is upregulated, affecting its interaction with other protein partners. This may set cells on a path to tumour formation. LMO4 and DEAF1 interact, but it is unknown how they cooperate to regulate cell proliferation. In this study, we identify a specific LMO4-binding domain in DEAF1. This domain contains an unstructured region that directly contacts LMO4, and a coiled coil that contains the DEAF1 nuclear export signal (NES). The coiled coil region can form tetramers and has the typical properties of a coiled coil domain. Using a simple cell-based assay, we show that LMO4 modulates the activity of the DEAF NES, causing nuclear accumulation of a construct containing the LMO4-interaction region of DEAF1.

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12 citations in Scopus
13 citations in Web of Science®
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ID Code: 55968
Item Type: Journal Article
Refereed: Yes
Keywords: DEAF1 protein, Structural domains, Breast cancer, Oncogene , LMO4 protein, mammary epithelial cell, cell proliferation
DOI: 10.1371/journal.pone.0039218
ISSN: 1932-6203
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > ONCOLOGY AND CARCINOGENESIS (111200) > Cancer Cell Biology (111201)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2012 Cubeddu et al.
Copyright Statement: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Deposited On: 21 Dec 2012 01:38
Last Modified: 07 Jan 2013 02:18

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