Cytokine synthesis by intestinal intraepithelial lymphocytes. Both gamma/delta T cell receptor-positive and alpha/beta T cell receptor-positive T cells in the G1 phase of cell cycle produce IFN-gamma and IL-5

Yamamoto, M., Fujihashi, K., Beagley, Ken W., McGhee, J. R., & Kiyono, H. (1993) Cytokine synthesis by intestinal intraepithelial lymphocytes. Both gamma/delta T cell receptor-positive and alpha/beta T cell receptor-positive T cells in the G1 phase of cell cycle produce IFN-gamma and IL-5. Journal of Immunology, 150(1), pp. 106-14.

Abstract

Murine intestinal intraepithelial lymphocytes (IEL) have been shown to contain subsets of alpha/beta TCR+ and gamma/delta TCR+ T cells that spontaneously produce cytokines such as IFN-gamma and IL-5. We have now determined the nature and cell cycle stage of these cytokine-producing T lymphocytes in EIL by using IFN-gamma- and IL-5-specific ELISPOT assay, cytokine-specific mRNA-cDNA dot-blot hybridization and polymerase chain reaction, and flow cytometry (FACS) for DNA analysis. When CD3+ T cells from IEL of normal C3H/HeN mice were separated into low and high density fractions by discontinuous Percoll gradients, IFN-gamma and IL-5 spot-forming cells were only found in the former population. Analysis of mRNA for these cytokines by both IFN-gamma- and IL-5-specific dot-blot hybridization and polymerase chain reaction revealed that higher levels of message for IFN-gamma and IL-5 were also seen in the low density fraction. However, cell cycle analysis of these two fractions by FACS using propidium iodide showed a similar pattern of cell cycle stages in both low and high density populations (G0 + G1 approximately 96 to 98% and S/G2 + M approximately 2 to 4%). Finally, mRNA from gamma/delta TCR+ and alpha/beta TCR+ T cells in both low and high density fractions of IEL were analyzed for IFN-gamma and IL-5 message by polymerase chain reaction. After 35 cycles of amplification, both gamma/delta TCR+ and alpha/beta TCR+ T cells in the low density fraction expressed higher levels of message for these two cytokines when compared with the high density population. These results have now shown that both gamma/delta and alpha/beta TCR+ IEL can be separated into low and high density subsets and both fractions possess a similar stage of cell cycle. However, only the low density cells (in G1 phase) of both gamma/delta and alpha/beta TCR types possess increased cytokine-specific mRNA and produce the cytokines IFN-gamma and IL-5. Our results suggest that alpha/beta TCR+ and gamma/delta TCR+ IEL can produce cytokines without cell proliferation.

Impact and interest:

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ID Code: 56319
Item Type: Journal Article
Refereed: Yes
Additional Information: Yamamoto, M
Fujihashi, K
Beagley, K W
McGhee, J R
Kiyono, H
AI 18958/AI/NIAID NIH HHS/United States
AI 19674/AI/NIAID NIH HHS/United States
DK 44240/DK/NIDDK NIH HHS/United States
Comparative Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
United states
Journal of immunology (Baltimore, Md. : 1950)
J Immunol. 1993 Jan 1;150(1):106-14.
Keywords: Animals, Epithelial Cells, Epithelium/immunology/metabolism, G1 Phase, Interferon-gamma/ biosynthesis, Interleukin-5/ biosynthesis, Intestinal Mucosa/cytology/ immunology/metabolism, Leukocyte Count, Mice, Mice, Inbred C3H, Receptors, Antigen, T-Cell, alpha-beta/ metabolism, Receptors, Antigen, T-Cell, gamma-delta/ metabolism, T-Lymphocytes/cytology/ metabolism
ISSN: 0022-1767 (Print) 0022-1767 (Linking)
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > IMMUNOLOGY (110700)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 1993 American Association of Immunologists
Deposited On: 10 Jan 2013 00:25
Last Modified: 17 Jul 2017 12:01

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