Enhanced proliferation, attachment and osteopontin expression of porcine periodontal cells with emdogain

Abstract

EmdogainW (EMD) is an enamel matrix derivative extracted from developing
porcine teeth with demonstrated periodontal regenerative potential. EMD has
been shown to influence a number of properties of periodontal ligament cells
including proliferation, cell attachment and matrix synthesis. To date, the effect
of EMD on the epithelial cell rests of Malassez (ERM) is unknown. In this study, periodontal ligament fibroblasts, ERM, alveolar bone cells and gingival
fibroblasts were obtained from porcine periodontal ligament, alveolar bone and
gingiva. This study investigated, in vitro, the effect of EMD at three concentrations
on proliferation, cell attachment and expression of mRNA for two mineralised tissuerelated proteins (osteopontin and bone sialoprotein). As for other periodontal cells, the ERM proliferative response was enhanced by EMD. Attachment assays revealed a highly significant increase for ERM and gingival fibroblasts after EMD treatment at all concentrations. This study has also shown that EMD stimulated expression of osteopontin mRNA by ERM and alveolar bone cells. The results from this study provide evidence that EMD enhanced cellular events related with proliferation, attachment and osteopontin mRNA expression by porcine periodontal cells, in a manner consistent with its role in periodontal regenerative therapy.