Enhanced proliferation, attachment and osteopontin expression of porcine periodontal cells with emdogain

Rincon, Julio, Xiao, Yin, Young, William G., & Bartold, P. Mark (2005) Enhanced proliferation, attachment and osteopontin expression of porcine periodontal cells with emdogain. Archives of Oral Biology, 50(12), pp. 1047-1054.

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EmdogainW (EMD) is an enamel matrix derivative extracted from developing porcine teeth with demonstrated periodontal regenerative potential. EMD has been shown to influence a number of properties of periodontal ligament cells including proliferation, cell attachment and matrix synthesis. To date, the effect of EMD on the epithelial cell rests of Malassez (ERM) is unknown. In this study, periodontal ligament fibroblasts, ERM, alveolar bone cells and gingival fibroblasts were obtained from porcine periodontal ligament, alveolar bone and gingiva. This study investigated, in vitro, the effect of EMD at three concentrations on proliferation, cell attachment and expression of mRNA for two mineralised tissuerelated proteins (osteopontin and bone sialoprotein). As for other periodontal cells, the ERM proliferative response was enhanced by EMD. Attachment assays revealed a highly significant increase for ERM and gingival fibroblasts after EMD treatment at all concentrations. This study has also shown that EMD stimulated expression of osteopontin mRNA by ERM and alveolar bone cells. The results from this study provide evidence that EMD enhanced cellular events related with proliferation, attachment and osteopontin mRNA expression by porcine periodontal cells, in a manner consistent with its role in periodontal regenerative therapy.

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ID Code: 5705
Item Type: Journal Article
Refereed: Yes
Additional Information: For more information, please refer to the journal’s website (see hypertext link) or contact the author.
Author contact details: yin.xiao@qut.edu.au
Keywords: Epithelial cell rests of Malassez, Osteopontin, RT, PCR, Emdogain, Attachment, Proliferation
DOI: 10.1016/j.archoralbio.2005.04.006
ISSN: 0003-9969
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)
Divisions: Past > QUT Faculties & Divisions > Faculty of Built Environment and Engineering
Copyright Owner: Copyright 2005 Elsevier
Deposited On: 25 Jul 2007 00:00
Last Modified: 18 Mar 2015 03:57

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