Quantifying the roles of cell motility and cell proliferation in a circular barrier assay

Simpson, Matthew J., Treloar, Katrina K., Binder, Benjamin J., Haridas, Parvathi, Manton, Kerry J., Leavesley, David I., McElwain, D. L. Sean, & Baker, Ruth E. (2013) Quantifying the roles of cell motility and cell proliferation in a circular barrier assay. Journal of the Royal Society. Interface, 10(82).

View at publisher

Abstract

Moving fronts of cells are essential features of embryonic development, wound repair and cancer metastasis. This paper describes a set of experiments to investigate the roles of random motility and proliferation in driving the spread of an initially confined cell population. The experiments include an analysis of cell spreading when proliferation was inhibited.

Our data have been analysed using two mathematical models: a lattice-based discrete model and a related continuum partial differential equation model. We obtain independent estimates of the random motility parameter, D, and the intrinsic proliferation rate, λ, and we confirm that these estimates lead to accurate modelling predictions of the position of the leading edge of the moving front as well as the evolution of the cell density profiles.

Previous work suggests that systems with a high λ/D ratio will be characterized by steep fronts, whereas systems with a low λ/D ratio will lead to shallow diffuse fronts and this is confirmed in the present study. Our results provide evidence that continuum models, based on the Fisher–Kolmogorov equation, are a reliable platform upon which we can interpret and predict such experimental observations.

Impact and interest:

35 citations in Scopus
Search Google Scholar™
31 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

22 since deposited on 21 Feb 2013
1 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 57433
Item Type: Journal Article
Refereed: Yes
Keywords: cell migration, cell proliferation, wound healing, cancer, mathematical modelling
DOI: 10.1098/rsif.2013.0007
ISSN: 1742-5689
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > Schools > School of Mathematical Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 The Royal Society Publishing
Deposited On: 21 Feb 2013 23:33
Last Modified: 03 Mar 2014 23:46

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page