Collection of Airborne Microorganisms into Liquid by Bubbling through Porous Medium
Agranovski, Igor E., Agranovski, Victoria, Grinshpun, Sergey A., Reponen, Tiina, & Willeke, Klaus (2002) Collection of Airborne Microorganisms into Liquid by Bubbling through Porous Medium. Aerosol Science and Technology, 36(4), pp. 502-509.
A new method for the removal of airborne particles by air bubbling through fibrous filters immersed into a liquid has recently been developed (Agranovski et al. 1999) and shown to be very efficient for cleaning air environments with ultra-fine aerosol particles. The principal objective of the present study was to evaluate the new bubbling technique for the collection of airborne bacteria into a liquid for subsequent physical and microbiological analysis. It was found that the technique is capable of achieving a physical collection efficiency of 98.5% or higher for particles larger than 0.3 wm in aerodynamic diameter. The physical collection efficiency of the prototype bubbler remained at that high level for 8 h of continuous operation with negligible variation of the pressure drop across the device. Evaporation of the collection fluid did not exceed 20% during 8 h, and the reaerosolization effect on the physical collection efficiency of the bubbler prototype was <8%. The recovery rate of gram-negative Pseudomonas fluorescens bacteria collected for 20 min was shown to be as high as 74% - 10%. Its decrease with time was not statistically significant: the recovery rate reached 63% - 15% and 58% - 16% after 4 and 8 h of continuous operation, respectively. Thus the bubbling technique was demonstrated to be suitable for collecting viable airborne bacteria even if they are sensitive to the stress.
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|Item Type:||Journal Article|
|Additional Information:||For more information, please refer to the journal’s website (see link) or contact the author. Author contact details: firstname.lastname@example.org|
|Subjects:||Australian and New Zealand Standard Research Classification > EARTH SCIENCES (040000) > ATMOSPHERIC SCIENCES (040100) > Atmospheric Sciences not elsewhere classified (040199)|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology|
|Copyright Owner:||Copyright 2002 Taylor & Francis|
|Copyright Statement:||First published in Aerosol Science and Technology 36(4):pp. 502-509.|
|Deposited On:||12 Dec 2006|
|Last Modified:||10 Aug 2011 22:58|
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