Development and evaluation of a new personal sampler for culturable airborne microorganisms
Agranovski, Igor E., Agranovski, Victoria, Reponen, Tiina, Willeke, Klaus, & Grinshpun, Sergey A. (2002) Development and evaluation of a new personal sampler for culturable airborne microorganisms. Atmospheric Environment, 36(5), pp. 889-898.
The objective of this study was to develop a new personal sampler for viable airborne microorganisms and to evaluate its performance under controlled laboratory conditions and in a field. In the sampler, air is bubbled through a porous medium submerged in a liquid layer, as has earlier been demonstrated to be highly efficient for air purification. The prototype had the physical collection efficiency >95% for particles >0.32 μm in aerodynamic diameter during 8 h of continuous operation. The pressure drop across the sampler was below 1700 Pa, much lower than that of most conventional bioaerosol samplers. The collection liquid losses due to evaporation and aerosolization did not exceed 18% in 8 h and the culturability of sampled microorganisms remained high: the recovery rate of stress-sensitive gram-negative P. fluorescens bacteria was 61±20%; for stress-resistant B. subtilis bacteria and A. versicolor fungal spores it was 95±9% and 97±6%, respectively. Six identical personal samplers were tested simultaneously on a simplified human manikin in an office environment. The culturable microbial concentration data obtained during 2, 4 and 8-h sampling were not affected by the sampling time. Inter-sample variation did not exceed 30%. The laboratory and field evaluations have demonstrated that the new sampler is capable of long-term personal sampling of airborne culturable microorganisms. The estimation of the detection limits has indicated that the sampler is capable of monitoring microbial exposure in the environments with the bacterial concentrations above 15 CFU/m3 and fungal concentrations above 5 CFU/m3 when using a sampling time of 8 h.
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|Item Type:||Journal Article|
|Additional Information:||For more information, please refer to the journal’s website (see link) or contact the author. Author contact details: email@example.com|
|Keywords:||Bioaerosol, Personal sampling, Viable microorganisms, Liquid collection, Collection efficiency|
|Subjects:||Australian and New Zealand Standard Research Classification > EARTH SCIENCES (040000) > ATMOSPHERIC SCIENCES (040100) > Atmospheric Sciences not elsewhere classified (040199)|
|Divisions:||Past > QUT Faculties & Divisions > Faculty of Science and Technology|
|Copyright Owner:||Copyright 2002 Elsevier|
|Deposited On:||12 Dec 2006|
|Last Modified:||10 Aug 2011 12:58|
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