Histological and morphometric lesions in the pre-clinical, developmental phase of insulin-induced laminitis in Standardbred horses
de Laat, Melody A., Patterson-Kane, Janet C., Pollitt, Christopher C., Sillence, Martin N., & McGowan, Catherine M. (2013) Histological and morphometric lesions in the pre-clinical, developmental phase of insulin-induced laminitis in Standardbred horses. The Veterinary Journal, 195(3), pp. 305-312.
Lamellar pathology in experimentally-induced equine laminitis associated with euglycaemic hyperinsulinaemia is substantial by the acute, clinical phase (∼48 h post-induction). However, lamellar pathology of the developmental, pre-clinical phase requires evaluation. The aim of this study was to analyse lamellar lesions both qualitatively and quantitatively, 6, 12 and 24 h after the commencement of hyperinsulinaemia. Histological and histomorphometrical analyses of lamellar pathology at each time-point included assessment of lamellar length and width, epidermal cell proliferation and death, basement membrane (BM) pathology and leucocyte infiltration. Archived lamellar tissue from control horses and those with acute, insulin-induced laminitis (48 h) was also assessed for cellular proliferative activity by counting the number of cells showing positive nuclear immuno labelling for TPX2.
Decreased secondary epidermal lamellar (SEL) width and increased histomorphological evidence of SEL epidermal basal (and supra-basal) cell death occurred early in disease progression (6 h). Increased cellular proliferation in SELs, infiltration of the dermis with small numbers of leucocytes and BM damage occurred later (24 and 48 h). Some lesions, such as narrowing of the SELs, were progressive over this time period (6–48 h). Cellular pathology preceded leucocyte infiltration and BM pathology, indicating that the latter changes may be secondary or downstream events in hyperinsulinaemic laminitis.
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|Item Type:||Journal Article|
|Keywords:||Equine, Laminitis, Hyperinsulinaemia, Histology, Epidermal lamellae, TPX2|
|Subjects:||Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000)|
|Divisions:||Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2012 Elsevier Ltd.|
|Copyright Statement:||NOTICE: this is the author’s version of a work that was accepted for publication in The Veterinary Journal. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in The Veterinary Journal, VOL 195, ISSUE 3, 2013. http://dx.doi.org/10.1016/j.tvjl.2012.07.003|
|Deposited On:||18 Mar 2013 22:01|
|Last Modified:||02 Apr 2014 11:17|
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