Effect of the sterilization method on the properties of Bombyx mori silk fibroin films

George, Karina A., Shadforth, Audra, Chirila, Traian V., Laurent, Matthieu, Stephenson, Sally-Anne, Edwards, Grant, Madden, Peter, Hutmacher, Dietmar, & Harkin, Damien G. (2013) Effect of the sterilization method on the properties of Bombyx mori silk fibroin films. Materials Science and Engineering C, 33(2), pp. 668-674.

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We have compared the effects of different sterilization techniques on the properties of Bombyx mori silk fibroin thin films with the view to subsequent use for corneal tissue engineering. The transparency, tensile properties, corneal epithelial cell attachment and degradation of the films were used to evaluate the suitability of certain sterilization techniques including gamma-irradiation (in air or nitrogen), steam treatment and immersion in aqueous ethanol. The investigations showed that gamma-irradiation, performed either in air or in a nitrogen atmosphere, did not significantly alter the properties of films. The films sterilized by gamma-irradiation or by immersion in ethanol had a transparency greater than 98% and tensile properties comparable to human cornea and amniotic membrane, the materials of choice in the reconstruction of ocular surface. Although steam-sterilization produced stronger, stiffer films, they were less transparent, and cell attachment was affected by the variable topography of these films. It was concluded that gamma-irradiation should be considered to be the most suitable method for the sterilization of silk fibroin films, however, the treatment with ethanol is also an acceptable method.

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8 citations in Web of Science®
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ID Code: 58509
Item Type: Journal Article
Refereed: Yes
Keywords: Silk fibroin, Sterilization, Tensile properties , Degradation , Cell adhesion
DOI: 10.1016/j.msec.2012.10.016
ISSN: 1873-0191
Subjects: Australian and New Zealand Standard Research Classification > ENGINEERING (090000) > BIOMEDICAL ENGINEERING (090300) > Biomaterials (090301)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300) > Ophthalmology (111301)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2012 Elsevier B.V.
Copyright Statement: NOTICE: this is the author’s version of a work that was accepted for publication in the journal, Materials Science and Engineering C. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Science and Engineering C, Volume 33, Issue 2, 1 March 2013, Pages 668–674, DOI: 10.1016/j.msec.2012.10.016
Deposited On: 21 Mar 2013 00:07
Last Modified: 01 Apr 2014 14:55

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