Modulation of LPS-induced chorioamnionitis by ureaplasma parvum in sheep chorioamnionitis in sheep

Snyder, Candice C., Wolfe, Katherine B., Gisslen, Tate, Knox, Christine L., Kemp, Matthew W., Kramer, Boris W., Newnham, John P., Jobe, Alan H., & Kallapur, Suhas G. (2013) Modulation of LPS-induced chorioamnionitis by ureaplasma parvum in sheep chorioamnionitis in sheep. American Journal of Obstetrics & Gynecology, 208(5), 399.e1-399.e8.

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Abstract

ABSTRACT Objective: Ureaplasma parvum colonization in the setting of polymicrobial flora is common in women with chorioamnionitis, and is a risk factor for preterm delivery and neonatal morbidity. We hypothesized that ureaplasma colonization of amniotic fluid will modulate chorioamnionitis induced by E.coli lipopolysaccharide (LPS).

Methods: Sheep received intra-amniotic (IA) injections of media (control) or live ureaplasma either 7 or 70d before delivery. Another group received IA LPS 2d before delivery. To test for interactions, U.parvum exposed animals were challenged with IA LPS, and delivered 2d later. All animals were delivered preterm at 125±1 day gestation.

Results: Both IA ureaplasmas and LPS induced leukocyte infiltration of chorioamnion. LPS greatly increased the expression of pro-inflammatory cytokines and myeloperoxidase in leukocytes, while ureaplasmas alone caused modest responses. Interestingly, 7d but not 70d ureaplasma exposure significantly downregulated LPS induced pro-inflammatory cytokines and myeloperoxidase expression in the chorioamnion.

Conclusion: U.parvum can suppress LPS induced experimental chorioamnionitis.

Impact and interest:

13 citations in Scopus
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1 citations in Web of Science®

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ID Code: 59117
Item Type: Journal Article
Refereed: Yes
Keywords: Ureaplasma species, Preterm labour, Fetal adaptation, Endotoxin, Innate immunity, Tolerance, Chorioamnionitis, lipopolysaccharide
DOI: 10.1016/j.ajog.2013.02.018
ISSN: 1097-6868 (online) 0002-9378 (print)
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > CLINICAL SCIENCES (110300) > Infectious Diseases (110309)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > PAEDIATRICS AND REPRODUCTIVE MEDICINE (111400)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2013 Mosby, Inc.
Copyright Statement: NOTICE: this is the author’s version of a work that was accepted for publication in American Journal of Obstetrics & Gynecology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in American Journal of Obstetrics & Gynecology, [Volume 208, Issue 5, (May 2013)] DOI: 10.1016/j.ajog.2013.02.018
Deposited On: 22 Apr 2013 23:45
Last Modified: 24 Jul 2015 08:38

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