SECIS-binding protein 2 promotes cell survival by protecting against oxidative stress

Papp, Laura V., Lu, Jun, Bolderson, Emma, Boucher, Didier, Singh, Ravindra, Holmgren, Arne, & Khanna, Kum Kum (2010) SECIS-binding protein 2 promotes cell survival by protecting against oxidative stress. Antioxidants & Redox Signaling, 12(7), pp. 797-808.

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Abstract

Reactive oxygen species (ROS) are a primary cause of cellular damage that leads to cell death. In cells, protection from ROS-induced damage and maintenance of the redox balance is mediated to a large extent by selenoproteins, a distinct family of proteins that contain selenium in form of selenocysteine (Sec) within their active site. Incorporation of Sec requires the Sec-insertion sequence element (SECIS) in the 3'-untranslated region of selenoproteins mRNAs and the SECIS-binding protein 2 (SBP2). Previous studies have shown that SBP2 is required for the Sec-incorporation mechanism; however, additional roles of SBP2 in the cell have remained undefined. We herein show that depletion of SBP2 by using antisense oligonucleotides (ASOs) causes oxidative stress and induction of caspase- and cytochrome c-dependent apoptosis. Cells depleted of SBP2 have increased levels of ROS, which lead to cellular stress manifested as 8-oxo-7,8-dihydroguanine (8-oxo-dG) DNA lesions, stress granules, and lipid peroxidation. Small-molecule antioxidants N-acetylcysteine, glutathione, and α-tocopherol only marginally reduced ROS and were unable to rescue cells fully from apoptosis, indicating that apoptosis might be directly mediated by selenoproteins. Our results demonstrate that SBP2 is required for protection against ROS-induced cellular damage and cell survival. Antioxid. Redox Signal. 12, 797–808.

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ID Code: 60135
Item Type: Journal Article
Refereed: Yes
Keywords: Oxidative stress, antioxidants, apoptosis
DOI: 10.1089/ars.2009.2913
ISSN: 1557-7716
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Biochemistry and Cell Biology not elsewhere classified (060199)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2010 Mary Ann Liebert Inc.
Copyright Statement: This is a copy of an article published in the Antioxidants & Redox Signaling © 2010 copyright Mary Ann Liebert, Inc.; Antioxidants & Redox Signaling is available online at: http://online.liebertpub.com.
Deposited On: 21 May 2013 01:01
Last Modified: 28 May 2013 00:48

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