Human single-stranded DNA binding proteins are essential for maintaining genomic stability

Ashton, Nicholas W., Bolderson, Emma, Cubeddu, Liza, O’Byrne, Kenneth J., & Richard, Derek J. (2013) Human single-stranded DNA binding proteins are essential for maintaining genomic stability. BMC Molecular Biology, 14, Article no. 9.

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Abstract

The double-stranded conformation of cellular DNA is a central aspect of DNA stabilisation and protection. The helix preserves the genetic code against chemical and enzymatic degradation, metabolic activation, and formation of secondary structures. However, there are various instances where single-stranded DNA is exposed, such as during replication or transcription, in the synthesis of chromosome ends, and following DNA damage. In these instances, single-stranded DNA binding proteins are essential for the sequestration and processing of single-stranded DNA. In order to bind single-stranded DNA, these proteins utilise a characteristic and evolutionary conserved single-stranded DNA-binding domain, the oligonucleotide/oligosaccharide-binding (OB)-fold. In the current review we discuss a subset of these proteins involved in the direct maintenance of genomic stability, an important cellular process in the conservation of cellular viability and prevention of malignant transformation. We discuss the central roles of single-stranded DNA binding proteins from the OB-fold domain family in DNA replication, the restart of stalled replication forks, DNA damage repair, cell cycle-checkpoint activation, and telomere maintenance.

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15 citations in Scopus
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14 citations in Web of Science®

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ID Code: 60136
Item Type: Journal Article
Refereed: Yes
Keywords: Single-stranded DNA binding proteins (SSBs), Oligonucleotide/oligosaccharide binding (OB)-fold, Double-strand DNA break (DSB) repair, Homology-directed repair (HDR), Translesion synthesis, Nucleotide excision repair (NER), Replication fork restart, Cell cycle checkpoint activation, Telomere maintenance
DOI: 10.1186/1471-2199-14-9
ISSN: 1471-2199
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Biochemistry and Cell Biology not elsewhere classified (060199)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2013 Ashton et al.
Copyright Statement: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Deposited On: 21 May 2013 01:28
Last Modified: 28 Apr 2016 22:35

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