Fecal indicators and bacterial pathogens in bottled water from Dhaka, Bangladesh

Ahmed, Warish, Yusuf, Rita, Hasan, Imtiaj, Ashraf, Warda, Goonetilleke, Ashantha, Toze, Simon, & Gardner, Ted (2013) Fecal indicators and bacterial pathogens in bottled water from Dhaka, Bangladesh. Brazilian Journal of Microbiology, 44(1), pp. 97-103.

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Forty-six bottled water samples representing 16 brands from Dhaka, Bangladesh were tested for the numbers of total coliforms, fecal indicator bacteria (i.e., thermotolerant Escherichia coli and Enterococcus spp.) and potential bacterial pathogens (i.e., Aeromonas hydrophil, Pseudomonas aeruginos, Salmonella spp., and Shigella spp.). Among the 16 brands tested, 14 (86%), ten (63%) and seven (44%) were positive for total coliforms, E. coil and Enterococcus spp., respectively. Additionally, a further nine (56%), eight (50%), six (37%), and four (25%) brands were PCR positive for A. hydrophila lip, P. aeruginosa ETA, Salmonella spp. invA, and Shigella spp. ipaH genes, respectively. The numbers of bacterial pathogens in bottled water samples ranged from 28 ± 12 to 600 ± 45 (A. hydrophila lip gene), 180 ± 40 to 900 ± 200 (Salmonella spp. invA gene), 180 ± 40 to 1,300 ± 400 (P. aeruginosa ETA gene) genomic units per L of water. Shigella spp. ipaH gene was not quantifiable. Discrepancies were observed in terms of the occurrence of fecal indicators and bacterial pathogens. No correlations were observed between fecal indicators numbers and presence/absence of A. hydrophila lip (p = 0.245), Salmonella spp. invA (p = 0.433), Shigella spp. ipaH gene (p = 0.078), and P. aeruginosa ETA (p = 0.059) genes. Our results suggest that microbiological quality of bottled waters sold in Dhaka, Bangladesh is highly variable. To protect public health, stringent quality control is recommended for the bottled water industry in Bangladesh.

Key words: bottled water, fecal indicator bacteria, quantitative PCR, bacterial pathogens, public health risk.

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ID Code: 61095
Item Type: Journal Article
Refereed: Yes
Keywords: bottled water, fecal indicator bacteria, quantitative PCR, bacterial pathogens, public health risk.
DOI: 10.1590/S1517-83822013005000026
ISSN: 1678-4405
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > MICROBIOLOGY (060500) > Microbiology not elsewhere classified (060599)
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright © 2013, Sociedade Brasileira de Microbiologia
Copyright Statement: All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC. http://creativecommons.org/licenses/by-nc/2.0/
Deposited On: 09 Jul 2013 02:42
Last Modified: 09 Jul 2013 23:01

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