Chitosan-collagen scaffolds with nano/microfibrous architecture for skin tissue engineering
Dey Sarkar, Soumi, Farrugia, Brooke L., Dargaville, Tim R., & Dhara, Santanu (2013) Chitosan-collagen scaffolds with nano/microfibrous architecture for skin tissue engineering. Journal of Biomedical Materials Research Part A, 101(12), pp. 3482-3492.
In this study, a hierarchical nano/microfibrous chitosan/collagen scaffold that approximates structural and functional attributes of native extracellular matrix (ECM), has been developed for applicability in skin tissue engineering. Scaffolds were produced by electrospinning of chitosan followed by imbibing of collagen solution, freeze-drying and subsequent cross-linking of two polymers. Scanning electron microscopy showed formation of layered scaffolds with nano/microfibrous architechture. Physico-chemical properties of scaffolds including tensile strength, swelling behavior and biodegradability were found satisfactory for intended application. 3T3 fibroblasts and HaCaT keratinocytes showed good in vitro cellular response on scaffolds thereby indicating the matrices′ cytocompatible nature. Scaffolds tested in an ex vivo human skin equivalent (HSE) wound model, as a preliminary alternative to animal testing, showed keratinocyte migration and wound re-epithelization — a pre-requisite for healing and regeneration. Taken together, the herein proposed chitosan/collagen scaffold, shows good potential for skin tissue engineering.
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|Item Type:||Journal Article|
|Keywords:||Nano/micro architecture, Chitosan, Collagen, Skin Tissue Engineering|
|Subjects:||Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > MACROMOLECULAR AND MATERIALS CHEMISTRY (030300) > Chemical Characterisation of Materials (030301)|
|Divisions:||Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
|Copyright Owner:||Copyright 2013 Wiley Periodicals, Inc.|
|Deposited On:||30 Jul 2013 23:05|
|Last Modified:||05 Jan 2015 10:02|
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