Chitosan-collagen scaffolds with nano/microfibrous architecture for skin tissue engineering

Dey Sarkar, Soumi, Farrugia, Brooke L., Dargaville, Tim R., & Dhara, Santanu (2013) Chitosan-collagen scaffolds with nano/microfibrous architecture for skin tissue engineering. Journal of Biomedical Materials Research Part A, 101(12), pp. 3482-3492.

View at publisher


In this study, a hierarchical nano/microfibrous chitosan/collagen scaffold that approximates structural and functional attributes of native extracellular matrix (ECM), has been developed for applicability in skin tissue engineering. Scaffolds were produced by electrospinning of chitosan followed by imbibing of collagen solution, freeze-drying and subsequent cross-linking of two polymers. Scanning electron microscopy showed formation of layered scaffolds with nano/microfibrous architechture. Physico-chemical properties of scaffolds including tensile strength, swelling behavior and biodegradability were found satisfactory for intended application. 3T3 fibroblasts and HaCaT keratinocytes showed good in vitro cellular response on scaffolds thereby indicating the matrices′ cytocompatible nature. Scaffolds tested in an ex vivo human skin equivalent (HSE) wound model, as a preliminary alternative to animal testing, showed keratinocyte migration and wound re-epithelization — a pre-requisite for healing and regeneration. Taken together, the herein proposed chitosan/collagen scaffold, shows good potential for skin tissue engineering.

Impact and interest:

14 citations in Scopus
Search Google Scholar™
14 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

34 since deposited on 30 Jul 2013
25 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 61587
Item Type: Journal Article
Refereed: Yes
Keywords: Nano/micro architecture, Chitosan, Collagen, Skin Tissue Engineering
DOI: 10.1002/jbm.a.34660
ISSN: 1552-4965
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > MACROMOLECULAR AND MATERIALS CHEMISTRY (030300) > Chemical Characterisation of Materials (030301)
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 Wiley Periodicals, Inc.
Deposited On: 30 Jul 2013 23:05
Last Modified: 05 Jan 2015 10:02

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page