Design and interpretation of cell trajectory assays

Bowden, Lucie, Simpson, Matthew, & Baker, Ruth (2013) Design and interpretation of cell trajectory assays. Journal of the Royal Society Interface, 10(88), pp. 1-12.

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Abstract

Cell trajectory data is often reported in the experimental cell biology literature to distinguish between different types of cell migration. Unfortunately, there is no accepted protocol for designing or interpreting such experiments and this makes it difficult to quantitatively compare different published data sets and to understand how changes in experimental design influence our ability to interpret different experiments. Here, we use an individual based mathematical model to simulate the key features of a cell trajectory experiment. This shows that our ability to correctly interpret trajectory data is extremely sensitive to the geometry and timing of the experiment, the degree of motility bias and the number of experimental replicates. We show that cell trajectory experiments produce data that is most reliable when the experiment is performed in a quasi 1D geometry with a large number of identically{prepared experiments conducted over a relatively short time interval rather than few trajectories recorded over particularly long time intervals.

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ID Code: 61592
Item Type: Journal Article
Refereed: Yes
Keywords: cell migration assay, cell trajectory, confidence, random walk model
DOI: 10.1098/rsif.2013.0630
ISSN: 1742-5662
Subjects: Australian and New Zealand Standard Research Classification > MATHEMATICAL SCIENCES (010000) > APPLIED MATHEMATICS (010200) > Biological Mathematics (010202)
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Current > Schools > School of Mathematical Sciences
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Funding:
Copyright Owner: Copyright 2013 The Royal Society
Deposited On: 31 Jul 2013 22:13
Last Modified: 03 Nov 2014 19:02

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