Matrix metalloproteinase biosensor based on a porous silicon reflector

Voelcker, Nico, Krismastuti, Fransiska, Cowin, Allison, Pace, Stephanie, Melville, Elisabeth, & Dargaville, Tim R. (2013) Matrix metalloproteinase biosensor based on a porous silicon reflector. Australian Journal of Chemistry, 66(11), pp. 1428-1434.

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Abstract

Matrix metalloproteinases (MMPs) are proteolytic enzymes important to wound healing. In non-healing wounds, it has been suggested that MMP levels become dysfunctional, hence it is of great interest to develop sensors to detect MMP biomarkers. This study presents the development of a label-free optical MMP biosensor based on a functionalised porous silicon (pSi) thin film. The biosensor is fabricated by immobilising a peptidomimetic MMP inhibitor in the porous layer using hydrosilylation followed by amide coupling. The binding of MMP to the immobilised inhibitor translates into a change of effective optical thickness (EOT) over the time. We investigate the effect of surface functionalisation on the stability of pSi surface and evaluate the sensing performance. We successfully demonstrate MMP detection in buffer solution and human wound fluid at physiologically relevant concentrations. This biosensor may find application as a point-of-care device that is prognostic of the healing trajectory of chronic wounds.

Impact and interest:

7 citations in Scopus
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8 citations in Web of Science®

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ID Code: 61889
Item Type: Journal Article
Refereed: Yes
DOI: 10.1071/CH13352
ISSN: 0004-9425
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > PHYSICAL CHEMISTRY (INCL. STRUCTURAL) (030600)
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 18 Aug 2013 23:02
Last Modified: 01 Sep 2014 05:27

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