Yersinia high pathogenicity island genes modify the Escherichia coli primary metabolome independently of siderophore production

Lv, Haitao & Henderson, Jeffrey P. (2011) Yersinia high pathogenicity island genes modify the Escherichia coli primary metabolome independently of siderophore production. Journal of Proteome Research, 10(12), pp. 5547-5554.

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Bacterial siderophores may enhance pathogenicity by scavenging iron, but their expression has been proposed to exert a substantial metabolic cost. Here we describe a combined metabolomic-genetic approach to determine how mutations affecting the virulence-associated siderophore yersiniabactin affect the Escherichia coli primary metabolome. Contrary to expectations, we did not find yersiniabactin biosynthesis to correspond to consistent metabolomic shifts. Instead, we found that targeted deletion of ybtU or ybtA, dissimilar genes with similar roles in regulating yersiniabactin expression, were associated with a specific shift in arginine pathway metabolites during growth in minimal media. This interaction was associated with high arginine levels in the model uropathogen Escherichia coli UTI89 compared to its ybtU and ybtA mutants and the K12 strain MG1655, which lacks yersiniabactin-associated genes. Because arginine is not a direct yersiniabactin biosynthetic substrate, these findings show that virulence-associated secondary metabolite systems may shape bacterial primary metabolism independently of substrate consumption

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8 citations in Web of Science®

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ID Code: 62033
Item Type: Journal Article
Refereed: Yes
Keywords: metabolomics, siderophore, yersiniabactin, Escherichia coli, primary metabolism, ybtU, ybtA, arginine biosynthesis
DOI: 10.1021/pr200756n
ISSN: 1535-3907
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 27 Aug 2013 03:19
Last Modified: 03 Sep 2013 02:13

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