Advantages of tandem LC-MS for the rapid assessment of tissue-specific metabolic complexity using a pentafluorophenylpropyl stationary phase

Lv, Haitao, Palacios, Gustavo, Hartil, Kirsten, & Kurland, Irwin J. (2011) Advantages of tandem LC-MS for the rapid assessment of tissue-specific metabolic complexity using a pentafluorophenylpropyl stationary phase. Journal of Proteome Research, 10(4), pp. 2104-2112.

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In this study, a tandem LC-MS (Waters Xevo TQ) MRM-based MS method was developed for rapid, broad profiling of hydrophilic metabolites from biological samples, in either positive or negative ion modes without the need for an ion pairing reagent, using a reversed-phase pentafluorophenylpropyl (PFPP) column. The developed method was successfully applied to analyze various biological samples from C57BL/6 mice, including urine, duodenum, liver, plasma, kidney, heart, and skeletal muscle. As result, a total 112 of hydrophilic metabolites were detected within 8 min of running time to obtain a metabolite profile of the biological samples. The analysis of this number of hydrophilic metabolites is significantly faster than previous studies. Classification separation for metabolites from different tissues was globally analyzed by PCA, PLS-DA and HCA biostatistical methods. Overall, most of the hydrophilic metabolites were found to have a "fingerprint" characteristic of tissue dependency. In general, a higher level of most metabolites was found in urine, duodenum, and kidney. Altogether, these results suggest that this method has potential application for targeted metabolomic analyzes of hydrophilic metabolites in a wide ranges of biological samples.

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ID Code: 62104
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Animals, Chromatography, Liquid/*instrumentation/*methods, *Metabolic Networks and Pathways, Metabolomics/*methods, Mice, Inbred C57BL, Multivariate Analysis, Silanes/chemistry, Tandem Mass Spectrometry/*instrumentation/*methods
DOI: 10.1021/pr1011119
ISSN: 1535-3907
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 02 Sep 2013 23:44
Last Modified: 05 Sep 2013 02:40

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