Metabolic urinary profiling of alcohol hepatotoxicity and intervention effects of Yin Chen Hao Tang in rats using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry

Wang, Xijun, Lv, Haitao, Sun, Hui, Liu, Lian, Yang, Bo, Sun, Wenjun, Wang, Ping, Zhou, Dixin, Zhao, Li, Dou, Shengshan, Zhang, Guangmei, & Cao, Hongxin (2008) Metabolic urinary profiling of alcohol hepatotoxicity and intervention effects of Yin Chen Hao Tang in rats using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 48(4), pp. 1161-1168.

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Abstract

This paper was designed to study metabonomic characters of the hepatotoxicity induced by alcohol and the intervention effects of Yin Chen Hao Tang (YCHT), a classic traditional Chinese medicine formula for treatment of jaundice and liver disorders in China. Urinary samples from control, alcohol- and YCHT-treated rats were analyzed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC/ESI-QTOF-MS) in positive ionization mode. The total ion chromatograms obtained from the control, alcohol- and YCHT-treated rats were easily distinguishable using a multivariate statistical analysis method such as the principal components analysis (PCA). The greatest difference in metabolic profiling was observed from alcohol-treated rats compared with the control and YCHT-treated rats. The positive ions m/z 664.3126 (9.00 min) was elevated in urine of alcohol-treated rats, whereas, ions m/z 155.3547 (10.96 min) and 708.2932 (9.01 min) were at a lower concentration compared with that in urine of control rats, however, these ions did not indicate a statistical difference between control rats and YCHT-treated rats. The ion m/z 664.3126 was found to correspond to ceramide (d18:1/25:0), providing further support for an involvement of the sphingomyelin signaling pathway in alcohol hepatotoxicity and the intervention effects of YCHT.

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ID Code: 62111
Item Type: Journal Article
Refereed: Yes
Keywords: Animals, Chromatography, High Pressure Liquid/*methods, Drug Toxicity, Drugs, Chinese Herbal/*metabolism, Ethanol/*toxicity, Hepatocytes/drug effects/pathology, Male, Medicine, Chinese Traditional/*methods, *Metabolome, Random Allocation, Rats, Wistar, Reproducibility of Results, Spectrometry, Mass, Electrospray Ionization/*methods, Urinalysis/methods, Urinary Tract/drug effects
DOI: 10.1016/j.jpba.2008.07.028
ISSN: 0731-7085
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 02 Sep 2013 23:57
Last Modified: 06 Sep 2013 00:48

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