Isolation and characterization of 21 polymorphic microsatellite loci in the iconic Australian lungfish, Neoceratodus forsteri, using the Ion Torrent next-generation sequencing platform

Huey, Joel A., Real, Kathryn M., Mather, Peter B., Chand, Vincent, Roberts, David T., Espinoza, Thomas, McDougall, Andrew, Kind, Peter K., Brooks, Steven, & Hughes, Jane M. (2013) Isolation and characterization of 21 polymorphic microsatellite loci in the iconic Australian lungfish, Neoceratodus forsteri, using the Ion Torrent next-generation sequencing platform. Conservation Genetics Resources, 5(3), pp. 737-740.

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Abstract

We isolated and characterized 21 microsatellite loci in the vulnerable and iconic Australian lungfish, Neoceratodus forsteri. Loci were screened across eight individuals from the Burnett River and 40 individuals from the Pine River. Genetic diversity was low with between one and six alleles per locus within populations and a maximum expected heterozygosity of 0.774. These loci will now be available to assess effective population sizes and genetic structure in N. forsteri across its natural range in South East Queensland, Australia.

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3 citations in Web of Science®

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ID Code: 62294
Item Type: Journal Article
Refereed: No
Keywords: Neoceratodus forsteri , Lungfish, Ion Torrent, Microsatellite
DOI: 10.1007/s12686-013-9894-8
ISSN: 1877-7260
Divisions: Current > Schools > School of Earth, Environmental & Biological Sciences
Current > Institutes > Institute for Future Environments
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: � Springer Science+Business Media Dordrecht 2013
Deposited On: 04 Sep 2013 05:15
Last Modified: 05 Sep 2013 01:01

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