Protective effects of sweroside on human MG-63 cells and rat osteoblasts

Sun, Hui, Li, Lijing , Zhang, Aihua , Zhang, Ning , Lu, Haitao , Sun, Wenjun , & Wang, Xijun (2013) Protective effects of sweroside on human MG-63 cells and rat osteoblasts. Fitoterapia, 84, pp. 174-179.

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Abstract

Herbal Fructus Corni is a folk medicine with a long history of safe use for treating osteoporosis in postmenopausal women or elderly men in Asia. Sweroside is a bioactive herbal ingredient isolated from Fructus Corni, which has been widely used for the treatment of osteoporosis in traditional Chinese medicine (TCM). Unfortunately, the working mechanisms of this compound are difficult to determine and thus remain unclear. The aim of the study was performed to determine the potential molecular mechanism of the anti-osteoporotic effect of sweroside on the human MG-63 cells and rat osteoblasts. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was used to observe the effect of sweroside on cell proliferation. The activity of alkaline phosphatase (ALP) and the amount of osteocalcin were also assayed the cell differentiation. Sweroside significantly increased the proliferation of human MG-63 cells and rat osteoblasts (P<0.01). It increased the activity of ALP, and osteocalcin was also elevated in response to sweroside (P<0.05). Of note, flowcytometer assay showed that sweroside can attenuate and inhibit apoptosis. Sweroside has a direct osteogenic effect on the proliferation and differentiation of cultured human MG-63 cells and rat osteoblasts in vitro. These data will help in understanding the molecular mechanisms of therapeutic efficacy of sweroside, and highlight insights into drug discovery. In the current study, sweroside has been suggested to be a promising osteoporosis therapeutic natural product.

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ID Code: 62304
Item Type: Journal Article
Refereed: Yes
Additional Information: Sun, Hui
Li, Lijing
Zhang, Aihua
Zhang, Ning
Lv, Haitao
Sun, Wenjun
Wang, Xijun
Netherlands
Fitoterapia. 2013 Jan;84:174-9. doi: 10.1016/j.fitote.2012.11.010. Epub 2012 Nov 28.
Keywords: Animals, Bone Density Conservation Agents/chemistry/*pharmacology, Cell Line, Cell Proliferation, Cornus/*chemistry, Humans, Iridoid Glucosides/chemistry/*pharmacology, Molecular Structure, Osteoblasts/*drug effects, Osteosarcoma, Rats, Rats, Wistar
DOI: 10.1016/j.fitote.2012.11.010
ISSN: 1873-6971 (Electronic)0367-326X (Linking)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 05 Sep 2013 01:55
Last Modified: 05 Sep 2013 21:51

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