Quality evaluation of Yin Chen Hao Tang extract based on fingerprint chromatogram and simultaneous determination of five bioactive constituents

Wang, Xijun, Lu, Haitao, Sun, Hui, Jiang, Xingang, Wu, Zeming, Sun, Wenjun, Wang, Ping, Liu, Lian, & Bi, Kaishun (2008) Quality evaluation of Yin Chen Hao Tang extract based on fingerprint chromatogram and simultaneous determination of five bioactive constituents. Journal of separation science, 31(1), pp. 9-15.

View at publisher


A completely validated method based on HPLC coupled with photodiode array detector (HPLC-UV) was described for evaluating and controlling quality of Yin Chen Hao Tang extract (YCHTE). First, HPLC-UV fingerprint chromatogram of YCHTE was established for preliminarily elucidating amount and chromatographic trajectory of chemical constituents in YCHTE. Second, for the first time, five mainly bioactive constituents in YCHTE were simultaneously determined based on fingerprint chromatogram for furthermore controlling the quality of YCHTE quantitatively. The developed method was applied to analyze 12 batches of YCHTE samples which consisted of herbal drugs from different places of production, showed acceptable linearity, intraday (RSD <5%), interday precision (RSD <4.80%), and accuracy (RSD <2.80%). As a result, fingerprint chromatogram determined 15 representative general fingerprint peaks, and the fingerprint chromatogram resemblances are all better than 0.9996. The contents of five analytes in different batches of YCHTE samples do not indicate significant difference. So, it is concluded that the developed HPLC-UV method is a more fully validated and complete method for evaluating and controlling the quality of YCHTE.

Impact and interest:

20 citations in Scopus
Search Google Scholar™
20 citations in Web of Science®

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

ID Code: 62306
Item Type: Journal Article
Refereed: Yes
Additional Information: Wang, Xijun
Lv, Haitao
Sun, Hui
Jiang, Xingang
Wu, Zeming
Sun, Wenjun
Wang, Ping
Liu, Lian
Bi, Kaishun
J Sep Sci. 2008 Jan;31(1):9-15.
Keywords: Fingerprint chromatogram, High-performance liquid chromatography, Quality control, Simultaneous determination, Yin Chen Hao Tang extract
DOI: 10.1002/jssc.200700376
ISSN: 1615-9306 (Print)1615-9306 (Linking)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 05 Sep 2013 03:15
Last Modified: 06 Sep 2013 01:41

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page