Simultaneous determination of 6,7-dimethylesculetin and geniposide in rat plasma and its application to pharmacokinetic studies of Yin Chen Hao Tang preparation

Wang, Xijun , Lu, Haitao , Sun, Hui , Sun, Wenjun , Liu, Lian , Wang, Ping , & Cao, Hongxin (2008) Simultaneous determination of 6,7-dimethylesculetin and geniposide in rat plasma and its application to pharmacokinetic studies of Yin Chen Hao Tang preparation. Arzneimittel-Forschung, 58(7), pp. 336-41.

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Abstract

A method for the rapid and simultaneous determination of 6,7-dimethylesculetin (CAS 120-08-1) and geniposide (CAS 24512-63-8) in rat plasma has been developed, using validated high performance liquid chromatography (HPLC) with solid phase extraction (SPE). The HPLC analysis was performed on a commercially available column (200 mm x 4.6 mm, 5 microm) with acetonitrile-methanol-0.1% aqueous formic acid as mobile phase and the UV detection at 343 nm and 238 nm for 6,7-dimethylesculetin and geniposide, respectively. The calibration curves for 6,7-dimethylesculetin and geniposide were linear over the range 0.4-25.6 microg/mL and 1.12-71.68 microg/mL, respectively. The lower limits of quantitation were 0.40 microg/ mL and 1.12 microg/mL, and the lower limits of detection were 0.06 microg/mL and 0.09 microg/ mL, respectively. The intra-day and inter-day precision for 6,7-dimethylesculetin and geniposide were < 5%, whereas the absolute recovery percentages were > 74%. A successful application of the developed HPLC analysis was demonstrated for the pharmacokinetic study of a Traditional Chinese Medicine formula of Yin Chen Hao Tang preparation.

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ID Code: 62307
Item Type: Journal Article
Refereed: Yes
Additional Information: Wang, Xijun
Lv, Haitao
Sun, Hui
Sun, Wenjun
Liu, Lian
Wang, Ping
Cao, Hongxin
Germany
Arzneimittelforschung. 2008;58(7):336-41. doi: 10.1055/s-0031-1296516.
Keywords: Algorithms, Animals, Calibration, Chromatography, High Pressure Liquid, Coumarins/*blood/*pharmacokinetics, Drugs, Chinese Herbal, Half-Life, Iridoids/*blood/*pharmacokinetics, Male, Rats, Rats, Wistar, Reproducibility of Results
DOI: 10.1055/s-0031-1296516
ISSN: 0004-4172 (Print)0004-4172 (Linking)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 05 Sep 2013 03:33
Last Modified: 06 Sep 2013 03:05

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