Development and validation of a ultra performance LC-ESI/MS method for analysis of metabolic phenotypes of healthy men in day and night urine samples

Wang, Xijun, Lv, Haitao, Zhang, Guangmei, Sun, Wenjun, Zhou, Dixin, Jiao, Guozheng, & Yu, Yang (2008) Development and validation of a ultra performance LC-ESI/MS method for analysis of metabolic phenotypes of healthy men in day and night urine samples. Journal of Separation Science, 31(16-17), pp. 2994-3001.

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Abstract

Ultra-performance LC coupled to quadrupole TOF/MS (UPLC-QTOF/MS) in positive and negative ESI was developed and validated to analyze metabolite profiles for urine from healthy men during the day and at night. Data analysis using principal components analysis (PCA) revealed differences between metabolic phenotypes of urine in healthy men during the day and at night. Positive ions with mass-to-charge ratio (m/z) 310.24 (5.35 min), 286.24 (4.74 min) and 310.24 (5.63 min) were elevated in the urine from healthy men at night compared to that during the day. Negative ions elevated in day urine samples of healthy men included m/z 167.02 (0.66 min), 263.12 (2.55 min) and 191.03 (0.73 min), whilst ions m/z 212.01 (4.77 min) were at a lower concentration in urine of healthy men during the day compared to that at night. The ions m/z 212.01 (4.77 min), 191.03 (0.73 min) and 310.24 (5.35 min) preliminarily correspond to indoxyl sulfate, citric acid and N-acetylneuraminic acid, providing further support for an involvement of phenotypic difference in urine of healthy men in day and night samples, which may be associated with notably different activities of gut microbiota, velocity of tricarboxylic acid cycle and activity of sialic acid biosynthesis in healthy men as regulated by circadian rhythm of the mammalian bioclock.

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ID Code: 62308
Item Type: Journal Article
Refereed: Yes
Additional Information: Wang, Xijun
Lv, Haitao
Zhang, Guangmei
Sun, Wenjun
Zhou, Dixin
Jiao, Guozheng
Yu, Yang
Germany
J Sep Sci. 2008 Sep;31(16-17):2994-3001. doi: 10.1002/jssc.200800326.
Keywords: Adult, Chromatography, Liquid/instrumentation/methods, *Circadian Rhythm, Citric Acid/metabolism/*urine, Citric Acid Cycle, Humans, Indican/metabolism/*urine, Male, N-Acetylneuraminic Acid/metabolism/*urine, Phenotype, Reference Values, Reproducibility of Results, Sensitivity and Specificity, Spectrometry, Mass, Electrospray Ionization/instrumentation/methods
DOI: 10.1002/jssc.200800326
ISSN: 1615-9314
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 04 Sep 2013 23:04
Last Modified: 06 Sep 2013 02:27

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