Effectiveness of cysteine proteases on protein/pigment film removal

Yao, Jiang-Wu, Xiao, Yin, Zuo, Qi-Liang, Zhang, Yi, Tao, Tao, & Lin, Chang-Jian (2013) Effectiveness of cysteine proteases on protein/pigment film removal. Archives of Oral Biology, 58(11), pp. 1618-1626.

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Abstract

Objective: Theaflavin (TF) from the black tea can react to human salivary proline-rich proteins (PRPs) to form stains on exposed dental surfaces. Here, we employed a model of protein/pigment film using TF and dephosphorylated bovine b-casein (Db-CN), which has an extended conformation, similar to that of salivary PRPs, on a sensor surface to assess the efficacy of cysteine proteases (CPs) including papain, stem bromelain, and ficin, on removing TF bound to Db-CN and the control TF readsorption on the residual substrate surfaces was also measured.

Methods: The protein/pigment complex film was built by using a quartz crystal microbalance with dissipation (QCM-D). The efficacies of CPs were assessed by Boltzman equation model. The surface details were detected by grazing angle infrared spectroscopy spectra, atomic force microscopy images, and contact angles.

Results: The efficacy order of CPs on hydrolyzing protein/pigment complex film is ficin > papain > bromelain. The results from grazing angle infrared spectroscopy spectra, atomic force microscopy images, and contact angles demonstrated that TF bound on the Db- CN was effectively removed by the CPs, and the amount of TF readsorption on both the residual film of the Db-CN/TF and the Db-CN was markedly decreased after hydrolysis.

Conclusion: This study indicates the potential application of the CPs for tooth stain removal and suggests that these enzymes are worthy of further investigation for use in oral healthcare.

Impact and interest:

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ID Code: 62484
Item Type: Journal Article
Refereed: Yes
Keywords: Cysteine proteases, Dephosphorylated b-casein, Theaflavin, Hydrolysis, Film
DOI: 10.1016/j.archoralbio.2013.07.015
ISSN: 0003-9969
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > DENTISTRY (110500)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 Elsevier Ltd.
Deposited On: 16 Sep 2013 06:11
Last Modified: 25 Sep 2013 21:08

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