Effect of pretreatment on saccharification of sugarcane bagasse by complex and simple enzyme mixtures

Harrison, Mark D., Zhang, Zhanying, Shand, Kylie, O'Hara, Ian M., Doherty, William O.S., & Dale, James L. (2013) Effect of pretreatment on saccharification of sugarcane bagasse by complex and simple enzyme mixtures. Bioresource Technology, 148, pp. 105-113.

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Abstract

Saccharification of sugarcane bagasse pretreated at the pilot-scale with different processes (in combination with steam-explosion) was evaluated. Maximum glucan conversion with Celluclast 1.5 L (15–25 FPU/g glucan) was in the following order: glycerol/HCl > HCl > H2SO4 > NaOH, with the glycerol system achieving ∼100% conversion. Surprisingly, the NaOH substrate achieved optimum saccharification with only 8 FPU/g glucan. Glucan conversions (3.6–6%) obtained with mixtures of endo-1,4-β-glucanase (EG) and β-glucosidase (βG) for the NaOH substrate were 2–6 times that of acid substrates. However, glucan conversions (15–60%) obtained with mixtures of cellobiohydrolase (CBH I) and βG on acidified glycerol substrate were 10–30% higher than those obtained for NaOH and acid substrates. The susceptibility of the substrates to enzymatic saccharification was explained by their physical and chemical attributes. Acidified glycerol pretreatment offers the opportunity to simplify the complexity of enzyme mixtures required for saccharification of lignocellulosics.

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13 citations in Scopus
14 citations in Web of Science®
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ID Code: 62529
Item Type: Journal Article
Refereed: Yes
Keywords: Pretreatment, Glycerol, Sugarcane, Bagasse, Cellulase
DOI: 10.1016/j.biortech.2013.08.099
ISSN: 1873-2976
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > OTHER CHEMICAL SCIENCES (039900) > Industrial Chemistry (039903)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Enzymes (060107)
Australian and New Zealand Standard Research Classification > TECHNOLOGY (100000) > INDUSTRIAL BIOTECHNOLOGY (100300) > Bioprocessing Bioproduction and Bioproducts (100302)
Divisions: Current > Research Centres > Centre for Tropical Crops and Biocommodities
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 Elsevier
Copyright Statement: This is the author’s version of a work that was accepted for publication in Bioresource Technology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Bioresource Technology, [VOL 148, (2013)] DOI: 10.1016/j.biortech.2013.08.099
Deposited On: 16 Sep 2013 22:07
Last Modified: 04 Nov 2015 01:26

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