Improved ultra-performance liquid chromatography with electrospray ionization quadrupole-time-of-flight high-definition mass spectrometry method for the rapid analysis of the chemical constituents of a typical medical formula: Liuwei Dihuang Wan

Wang, Ping, Lv, Haitao, Zhang, Ai Hua, Sun, Hui, Yan, Guangli, Han, Ying, Wu, Xiu Hong, & Wang, Xi Jun (2013) Improved ultra-performance liquid chromatography with electrospray ionization quadrupole-time-of-flight high-definition mass spectrometry method for the rapid analysis of the chemical constituents of a typical medical formula: Liuwei Dihuang Wan. Journal of Separation Science, 36(21-22), pp. 3511-3516.

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Abstract

Liuwei Dihuang Wan (LWD), a classic Chinese medicinal formulae, has been used to improve or restore declined functions related to aging and geriatric diseases, such as impaired mobility, vision, hearing, cognition and memory. It has attracted increasingly much attention as one of the most popular and valuable herbal medicines. However, the systematic analysis of the chemical constituents of LDW is difficult and thus has not been well established. In this paper, a rapid, sensitive and reliable ultra-performance liquid chromatography with electrospray ionization quadrupole time-of-flight high-definition mass spectrometry (UPLC-ESI-Q-TOF-MS) method with automated MetaboLynx analysis in positive and negative ion mode was established to characterize the chemical constituents of LDW. The analysis was performed on a Waters UPLCTM HSS T3 using a gradient elution system. MS/MS fragmentation behavior was proposed for aiding the structural identification of the components. Under the optimized conditions, a total of 50 peaks were tentatively characterized by comparing the retention time and MS data. It is concluded that a rapid and robust platform based on UPLC-ESI-Q-TOF-MS has been successfully developed for globally identifying multiple-constituents of traditional Chinese medicine prescriptions. This is the first report on systematic analysis of the chemical constituents of LDW. This article is protected by copyright. All rights reserved.

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ID Code: 62662
Item Type: Journal Article
Refereed: Yes
Additional Information: Wang, Ping
Lv, Hai Tao
Zhang, Ai Hua
Sun, Hui
Yan, Guang Li
Han, Ying
Wu, Xiu Hong
Wang, Xi Jun
J Sep Sci. 2013 Aug 29. doi: 10.1002/jssc.201300742.
DOI: 10.1002/jssc.201300742
ISSN: 1615-9314
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 18 Sep 2013 23:19
Last Modified: 02 Mar 2014 22:35

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