Matrix metalloproteinase localisation by in situ-RT-PCR in archival human breast biopsy material

Haupt, Larisa M., Irving, Rachel E., Weinstein, Stephen R., Irving, Michael G., & Griffiths, Lyn R. (2008) Matrix metalloproteinase localisation by in situ-RT-PCR in archival human breast biopsy material. Molecular and Cellular Probes, 22(2), pp. 83-89.

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Utilising archival human breast cancer biopsy material we examined the stromal/epithelial interactions of several matrix metalloproteinases (MMPs) using in situ-RT-PCR (IS-RT-PCR). In breast cancer, the stromal/epithelial interactions that occur, and the site of production of these proteases, are central to understanding their role in invasive and metastatic processes. We examined MT1-MMP (MMP-14, membrane type-1-MMP), MMP-1 (interstitial collagenase) and MMP-3 (stromelysin-1) for their localisation profile in progressive breast cancer biopsy material (poorly differentiated invasive breast carcinoma (PDIBC), invasive breast carcinomas (IBC) and lymph node metastases (LNM)). Expression of MT1-MMP, MMP-1 and MMP-3 was observed in both the tumour epithelial and surrounding stromal cells in most tissue sections examined. MT1-MMP expression was predominantly localised to the tumour component in the pre-invasive lesions. MMP-1 gene expression was relatively well distributed between both tissue compartments, while MMP-3 demonstrated highest expression levels in the stromal tissue surrounding the epithelial tumour cells. The results demonstrate the ability to distinguish compartmental gene expression profiles using IS-RT-PCR. Further, we suggest a role for MT1-MMP in early tumour progression, expression of MMP-1 during metastasis and focal expression pattern of MMP-3 in areas of expansion. These expression profiles may provide markers for early breast cancer diagnoses and present potential therapeutic targets.

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ID Code: 62682
Item Type: Journal Article
Refereed: Yes
DOI: 10.1016/j.mcp.2007.06.009
ISSN: 1096-1194
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2008 Academic Press
Deposited On: 19 Sep 2013 05:34
Last Modified: 07 Apr 2014 00:04

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