A genetic analysis of serotonergic biosynthetic and metabolic enzymes in migraine using a DNA pooling approach

Johnson, Matthew P. & Griffiths, Lyn R. (2005) A genetic analysis of serotonergic biosynthetic and metabolic enzymes in migraine using a DNA pooling approach. Journal of Human Genetics, 50(12), pp. 607-610.

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Abstract

Migraine is a common debilitating primary headache disorder with significant mental, physical and social health implications. The brain neurotransmitter 5-hydroxytryptamine (5-HT; serotonin) is involved in nociceptive pathways and has been implicated in the pathophysiology of migraine. With few genetic studies investigating biosynthetic and metabolic enzymes governing the rate of 5-HT activity and their relationship to migraine, it was the objective of this study to assess genetic variants within the human tryptophan hydroxylase (TPH), amino acid decarboxylase (AADC) and monoamine oxidase A (MAOA) genes in migraine susceptibility. This objective was undertaken using a high-throughput DNA pooling experimental design, which proved to be a very accurate, sensitive and specific method of estimating allele frequencies for single nucleotide polymorphism, insertion deletion and variable number tandem repeat loci. Application of DNA pooling to a wide array of genetic loci provides greater scope in the assessment of population-based genetic association study designs. Despite the application of this high-throughput genotyping method, negative results from the two-stage DNA pooling design used to screen loci within the TPH, AADC and MAOA genes did not support their role in migraine susceptibility.

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18 citations in Web of Science®

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ID Code: 62728
Item Type: Journal Article
Refereed: Yes
Keywords: Migraine; Serotonin; DNA pooling; Genetic Association
DOI: 10.1007/s10038-005-0301-5
ISSN: 1435-232X
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2005 the authors
Deposited On: 20 Sep 2013 02:18
Last Modified: 07 Nov 2013 03:11

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