SERS based detection of barcoded gold nanoparticle assemblies from within animal tissue

Dey, Priyanka, Olds, William J., Blakey, Idriss, Thurecht, Kristofer, Izake, Emad L., & Fredericks, Peter M. (2013) SERS based detection of barcoded gold nanoparticle assemblies from within animal tissue. Journal of Raman Spectroscopy, 44(12), pp. 1659-1665.

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We have explored the potential of deep Raman spectroscopy, specifically surface enhanced spatially offset Raman spectroscopy (SESORS), for non-invasive detection from within animal tissue, by employing SERS-barcoded nanoparticle (NP) assemblies as the diagnostic agent. This concept has been experimentally verified in a clinic-relevant backscattered Raman system with an excitation line of 785 nm under ex vivo conditions. We have shown that our SORS system, with a fixed offset of 2-3 mm, offered sensitive probing of injected QTH-barcoded NP assemblies through animal tissue containing both protein and lipid. In comparison to that of non-aggregated SERS-barcoded gold NPs, we have demonstrated that the tailored SERS-barcoded aggregated NP assemblies have significantly higher detection sensitivity. We report that these NP assemblies can be readily detected at depths of 7-8 mm from within animal proteinaceous tissue with high signal-to-noise (S/N) ratio. In addition they could also be detected from beneath 1-2 mm of animal tissue with high lipid content, which generally poses a challenge due to high absorption of lipids in the near-infrared region. We have also shown that the signal intensity and S/N ratio at a particular depth is a function of the SERS tag concentration used and that our SORS system has a QTH detection limit of 10-6 M. Higher detection depths may possibly be obtained with optimization of the NP assemblies, along with improvements in the instrumentation. Such NP assemblies offer prospects for in vivo, non-invasive detection of tumours along with scope for incorporation of drugs and their targeted and controlled release at tumour sites. These diagnostic agents combined with drug delivery systems could serve as a “theranostic agent”, an integration of diagnostics and therapeutics into a single platform.

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7 citations in Scopus
6 citations in Web of Science®
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ID Code: 62748
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: SESORS, nanoparticle assemblies, SERS barcoding, diagnostic agent, deep tissue detection
DOI: 10.1002/jrs.4399
ISSN: 0377-0486
Subjects: Australian and New Zealand Standard Research Classification > CHEMICAL SCIENCE (030000) > MACROMOLECULAR AND MATERIALS CHEMISTRY (030300) > Nanochemistry and Supramolecular Chemistry (030302)
Divisions: Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 John Wiley & Sons
Deposited On: 22 Sep 2013 23:23
Last Modified: 02 Jun 2014 06:15

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