Identification and simultaneous analysis of harmane, harmol, isovitexin and vitexin in Passiflora incarnata extracts with a novel HLPC method

Grice, I. D., Ferreira, L. A., & Griffiths, Lyn R. (2001) Identification and simultaneous analysis of harmane, harmol, isovitexin and vitexin in Passiflora incarnata extracts with a novel HLPC method. Journal of Liquid Chromatography & Related Technologies, 24(16), pp. 2513-2523.

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Abstract

A high performance liquid chromatographic method for the simultaneous analysis of two flavonoids (iso-vitexin and vitexin), and three indole alkaloids (harmane, harmine, and harmol) was developed. This method was then utilised to quantitate levels of these five constituents in methanolic extracts of Australian Passiflora incarnata.

HPLC analysis was performed using a Waters™ Novapak C18 (150 × 4 mm, 4 μm) column, with a gradient solvent system of methanol-water-acetic acid. Detection was achieved by PDA UV (254 nm) and fluorescence (excitation 254 nm, emission 414 nm), utilising the external standard method to obtain quantification.

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8 citations in Web of Science®

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ID Code: 62824
Item Type: Journal Article
Refereed: Yes
DOI: 10.1081/JLC-100105957
ISSN: 1520-572X
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2001 Taylor & Francis Inc.
Deposited On: 24 Sep 2013 02:49
Last Modified: 24 Sep 2013 02:49

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