An improved method for the assay of platelet pyruvate dehydrogenase

Schofield, P. J., Griffiths, Lyn R., Rogers, Susan H., & Wise, G. (1980) An improved method for the assay of platelet pyruvate dehydrogenase. Clinica Chimica Acta, 108(2), pp. 219-227.

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Abstract

An improved method for the assay of human platelet pyruvate dehydrogenase is described. By generating the substrate [1-14C]pyruvate in situ from [1-14C]lactate plus l-lactate dehydrogenase, the rate of spontaneous decarboxylation is dramatically reduced, allowing far greater sensitivity in the assay of low activities of pyruvate dehydrogenase. In addition, no special precautions are required for the storage and use of [1-14C]lactate, in contrast to those for [1-14C] pyruvate. These factors allow a 5–10-fold increase in sensitivity compared with current methods. The pyruvate dehydrogenase activity of normal subjects as determined by the [1-14C]lactate system was 215 ± 55 pmol · min−1 · mg−1 protein (n = 18). The advantages of this assay system are discussed.

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17 citations in Scopus
22 citations in Web of Science®
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ID Code: 62948
Item Type: Journal Article
Refereed: Yes
DOI: 10.1016/0009-8981(80)90008-X
ISSN: 0009-8981
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 1980 Elsevier BV
Deposited On: 26 Sep 2013 04:33
Last Modified: 26 Sep 2013 04:33

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