Pten and Ndufb8 aberrations in cervical cancer tissue

Hsieh, S. M., Maguire, David J., Lintell, Nicholas A., McCabe, Michael, & Griffiths, Lyn R. (2008) Pten and Ndufb8 aberrations in cervical cancer tissue. Advances in Experimental Medicine and Biology, 599, pp. 31-36.

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Abstract

Cervical cancer is one of the world's major health issues. Despite many studies in this field, the carcinogenetic events of malignant conversion in cervical tumours have not been significantly characterised. The first aim of this project was to investigate the mutation status of the tumour suppressor gene- Phosphatase and Tension Homolog (PTEN)- in cervical cancer tissue. The second aim of this study was the analysis in the same cervical cancer tissue for aberrations in the mitochondrial electron transport chain subunit gene NDUFB8, which is localised to the same chromosomal contig as PTEN. The third aim was the evaluation of the potential therapeutic anti-cancer drug 2,4-Thiazolidinediones (TZDs) and its affect in regulating the PTEN protein in a cervical cancer cell line (HeLa). To approach the aims, paraffin-embedded cancerous cervical tissue and non-cancerous cervical tissue were obtained. DNA recovered from those tissues was then used to investigate the putative genomic changes regarding the NDUFB8 gene utilising SYBR Green I Real-Time PCR. The PTEN gene was studied via Dual-Labelled probe Real-Time PCR. To investigate the protein expression change of the PTEN protein, HeLa cells were firstly treated with different concentrations of 2,4-Thiazolidinediones and the level of PTEN protein expression was then observed utilising standard protein assays. Results indicated that there were putative copy-number changes between the cancerous cervical tissue and non-cancerous cervical tissue, with regard to the PTEN locus. This implies a potential gain of the PTEN gene in cancerous cervical tissue. With regards to normal cervical tissue versus cancerous cervical tissue no significant melting temperature differences were observed with the SYBR Green I Real-Time PCR in respect to the NDUFB8 gene. A putative up-regulation of PTEN protein was observed in TZD treated HeLa cells. © 2008 Springer Science+Business Media, LLC.

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ID Code: 63126
Item Type: Journal Article
Refereed: Yes
Additional Information: 17727244
Keywords: 2, 4 thiazolidinedione, alpha tubulin, genomic DNA, phosphatidylinositol 3, 4, 5 trisphosphate 3 phosphatase, 2, 4 thiazolidinedione derivative, antineoplastic agent, multienzyme complex, phosphatidylinositol 3, 4, 5 trisphosphate 3 phosphatase, protein subunit, PTEN protein, human, unclassified drug, cancer tissue, concentration response, conference paper, controlled study, drug mechanism, gene, HeLa cell, human, human cell, human tissue, mitochondrial gene, ndufb 8 gene, numerical chromosome aberration, priority journal, protein expression, PTEN gene, real time polymerase chain reaction, temperature sensitivity, upregulation, uterine cervix cancer, article, female, genetics, metabolism, pathology, uterine cervix tumor, Antineoplastic Agents, Electron Transport Chain Complex Proteins, Female, Hela Cells, Humans, Protein Subunits, PTEN Phosphohydrolase, Thiazolidinediones, Uterine Cervical Neoplasms
DOI: 10.1007/978-0-387-71764-7_5
ISSN: 0065-2598
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2008 Springer New York LLC
Deposited On: 04 Oct 2013 00:28
Last Modified: 11 Nov 2013 03:46

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