A linkage and cross-sectional study of hypertension and obesity using a poly(A) Alu-repeat polymorphism at the glucagon receptor gene locus (17q25)

Rutherford, S., Boatwright, S. D., Samwell, G. A., Morris, B. J., & Griffiths, Lyn R. (1998) A linkage and cross-sectional study of hypertension and obesity using a poly(A) Alu-repeat polymorphism at the glucagon receptor gene locus (17q25). Clinical and Experimental Pharmacology and Physiology, 25(7-Aug), pp. 627-629.

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  1. Previous glucagon receptor gene (GCGR) studies have shown a Gly40Ser mutation to be more prevalent in essential hypertension and to affect glucagon binding affinity to its receptor. An Alu-repeat poly(A) polymorphism colocalized to GCGR was used in the present study to test for association and linkage in hypertension as well as association in obesity development. 2. Using a cross-sectional approach, 85 hypertensives and 95 normotensives were genotyped using polymerase chain reaction primers flanking the Alu-repeat. Both hypertensive and normotensive populations were subdivided into lean and obese categories based on body mass index (BMI) to determine involvement of this variant in obesity. For the linkage study, 89 Australian Caucasian hypertension affected sibships (174 sibpairs) were genotyped and the results were analysed using GENE-HUNTER, Mapmaker Sibs, ERPA and SPLINK (all freely available from http://linlkage.rockefeller. edu/soft/list.html). 3. Cross-sectional results for both hypertension and obesity were analysed using Chi-squared and Monte Carlo analyses. Results did not show an association of this variant with either hypertension (χ2 = 6.9, P = 0.14; Monte Carlo χ2 = 7.0, P = 0.11; n = 5000) or obesity (χ2 = 3.3, P = 0.35; Monte Carlo χ2 = 3.26, P = 0.34; n = 5000). In addition, results from the linkage study using hypertensive sib-pairs did not indicate linkage of the poly(A) repent with hypertension. Hence, results did not indicate a role far the Alu-repeat in either hypertension or obesity. However, as the heterozygosity of this poly(A) repeat is low (35%), a larger number of hypertensive sib-pairs may be required to draw definitive conclusions.

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5 citations in Web of Science®
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ID Code: 63188
Item Type: Journal Article
Refereed: Yes
Additional Information: 9673441
Keywords: Association studies, Hypertensiion, Linkage studies, Molecular genetics, Obesity, Polymerase chain reaction, Polymorphism, glucagon receptor, adult, binding affinity, body mass, clinical trial, conference paper, dna polymorphism, female, gene locus, genotype, human, hypertension, major clinical study, male, molecular genetics, mutation, obesity, polymerase chain reaction, statistics, Diabetes Mellitus, Type 2, DNA, Genetic Markers, Humans, Hyperlipidemias, Hypertension, Linkage (Genetics), Microvascular Angina, Middle Aged, Obesity, Poly A, Polymorphism, Genetic, Receptors, Glucagon, Repetitive Sequences, Nucleic Acid
ISSN: 0305-1870
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 1998 Wiley-Blackwell Publishing Asia
Deposited On: 08 Oct 2013 02:15
Last Modified: 08 Oct 2013 02:15

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