Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue.

Smith, R. A., Lea, Rod A., Curran, J. E., Weinstein, Stephen R., & Griffiths, Lyn R. (2002) Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue. Breast Cancer Research, 5(1), R9-12.

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BACKGROUND: Previous studies in our laboratory have shown associations of specific nuclear receptor gene variants with sporadic breast cancer. In order to investigate these findings further, we conducted the present study to determine whether expression levels of the progesterone and glucocorticoid nuclear receptor genes vary in different breast cancer grades. METHODS: RNA was extracted from paraffin-embedded archival breast tumour tissue and converted into cDNA. Sample cDNA underwent PCR using labelled primers to enable quantitation of mRNA expression. Expression data were normalized against the 18S ribosomal gene multiplex and analyzed using analysis of variance. RESULTS: Analysis of variance indicated a variable level of expression of both genes with regard to breast cancer grade (P = 0.00033 for glucocorticoid receptor and P = 0.023 for progesterone receptor). CONCLUSION: Statistical analysis indicated that expression of the progesterone nuclear receptor is elevated in late grade breast cancer tissue.

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13 citations in Web of Science®

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ID Code: 63193
Item Type: Journal Article
Refereed: Yes
Additional Information: 12559052
Keywords: cell receptor, glucocorticoid receptor, progesterone receptor, RNA, article, breast tumor, female, gene expression regulation, genetics, human, metabolism, middle aged, Breast Neoplasms, Female, Gene Expression Regulation, Neoplastic, Humans, Middle Aged, Receptors, Cytoplasmic and Nuclear, Receptors, Glucocorticoid, Receptors, Progesterone, RNA
ISSN: 1465-542X
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2002 BioMed Central
Deposited On: 08 Oct 2013 03:02
Last Modified: 26 Jul 2014 04:08

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