Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue.

Smith, R. A., Lea, Rod A., Curran, J. E., Weinstein, Stephen R., & Griffiths, Lyn R. (2002) Expression of glucocorticoid and progesterone nuclear receptor genes in archival breast cancer tissue. Breast Cancer Research, 5(1), R9-12.

View at publisher (open access)

Abstract

BACKGROUND: Previous studies in our laboratory have shown associations of specific nuclear receptor gene variants with sporadic breast cancer. In order to investigate these findings further, we conducted the present study to determine whether expression levels of the progesterone and glucocorticoid nuclear receptor genes vary in different breast cancer grades. METHODS: RNA was extracted from paraffin-embedded archival breast tumour tissue and converted into cDNA. Sample cDNA underwent PCR using labelled primers to enable quantitation of mRNA expression. Expression data were normalized against the 18S ribosomal gene multiplex and analyzed using analysis of variance. RESULTS: Analysis of variance indicated a variable level of expression of both genes with regard to breast cancer grade (P = 0.00033 for glucocorticoid receptor and P = 0.023 for progesterone receptor). CONCLUSION: Statistical analysis indicated that expression of the progesterone nuclear receptor is elevated in late grade breast cancer tissue.

Impact and interest:

13 citations in Web of Science®
Search Google Scholar™

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

16 since deposited on 08 Oct 2013
1 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 63193
Item Type: Journal Article
Refereed: Yes
Additional Information: 12559052
Keywords: cell receptor, glucocorticoid receptor, progesterone receptor, RNA, article, breast tumor, female, gene expression regulation, genetics, human, metabolism, middle aged, Breast Neoplasms, Female, Gene Expression Regulation, Neoplastic, Humans, Middle Aged, Receptors, Cytoplasmic and Nuclear, Receptors, Glucocorticoid, Receptors, Progesterone, RNA
ISSN: 1465-542X
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2002 BioMed Central
Deposited On: 08 Oct 2013 03:02
Last Modified: 26 Jul 2014 04:08

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page