Ethanol exposure alters monoamine oxidase gene-expression in neuronal SH-SY5Y cells
Green, Michael R., Miller, John H., Griffiths, Lyn R., & Lea, Rod A. Ethanol exposure alters monoamine oxidase gene-expression in neuronal SH-SY5Y cells. [Working Paper] (Unpublished)
Abstract: Monoamine Oxidase (MAO) enzymes catabolise, and thus modulate abundance of, neurotransmitters in the brain. Variation in MAO enzyme activity has been linked to alcohol abuse behaviour, although the molecular mechanisms underlying this association are not understood. The present study evaluated relative gene-transcript abundance of MAO-A and MAO-B in the SH-SY5Y human neuroblastoma cell-line in response to ethanol exposure and following ethanol withdrawal. We found that each isoform of MAO was significantly transcriptionally up-regulated 55-80% in response to 100mM ethanol exposure. This trend was maintained following prolonged exposures (24 h-72 h) and with short exposures (24 h) followed by a period of ethanol withdrawal, suggesting that the transcriptional regulation is the result of a cellular change occurring within the first 24 hours of ethanol exposure. These results suggest a role for MAO transcriptional regulation in the complex neurobiochemical changes underlying alcohol addiction.
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|Item Type:||Working Paper|
|Divisions:||Current > Institutes > Institute of Health and Biomedical Innovation|
|Copyright Owner:||Copyright the authors.|
|Deposited On:||23 Oct 2013 02:57|
|Last Modified:||23 Oct 2013 04:36|
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