Ethanol exposure alters monoamine oxidase gene-expression in neuronal SH-SY5Y cells

Green, Michael R., Miller, John H., Griffiths, Lyn R., & Lea, Rod A. Ethanol exposure alters monoamine oxidase gene-expression in neuronal SH-SY5Y cells. [Working Paper] (Unpublished)

Abstract

Abstract: Monoamine Oxidase (MAO) enzymes catabolise, and thus modulate abundance of, neurotransmitters in the brain. Variation in MAO enzyme activity has been linked to alcohol abuse behaviour, although the molecular mechanisms underlying this association are not understood. The present study evaluated relative gene-transcript abundance of MAO-A and MAO-B in the SH-SY5Y human neuroblastoma cell-line in response to ethanol exposure and following ethanol withdrawal. We found that each isoform of MAO was significantly transcriptionally up-regulated 55-80% in response to 100mM ethanol exposure. This trend was maintained following prolonged exposures (24 h-72 h) and with short exposures (24 h) followed by a period of ethanol withdrawal, suggesting that the transcriptional regulation is the result of a cellular change occurring within the first 24 hours of ethanol exposure. These results suggest a role for MAO transcriptional regulation in the complex neurobiochemical changes underlying alcohol addiction.

Impact and interest:

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

55 since deposited on 23 Oct 2013
3 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 63664
Item Type: Working Paper
Refereed: No
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright the authors.
Deposited On: 23 Oct 2013 02:57
Last Modified: 23 Oct 2013 04:36

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page