The role of EBV-encoded microRNAs in B cell differentiation

Ross, Nathan Arlen (2012) The role of EBV-encoded microRNAs in B cell differentiation. Masters by Research thesis, Queensland University of Technology.


Epstein Barr virus (EBV) is a common γ-herpes virus, infecting approximately 90% of the world‟s population. It is also one of the first known viruses known to be oncogenic, and is associated with a number of tumour types, primarily lymphomas.

MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression and many human miRNAs have been associated with the development of malignancies including cancer. EBV was the first human virus identified to express miRNAs and encodes more than 40 miRNAs within its genome. Yet, an understanding of the targets of EBV-miRNAs, and thereby the function of them in pathogenesis remains sadly limited.

This study identifies a potential novel target of EBV-miRNAs, MECP2 and characterises the miRNA:mRNA interactions between two previously identified novel targets; Bim and EBF1. In particular, this study focuses upon the interaction between EBF1 and the EBV-miRNA BART11-5p, demonstrating a 151bp region of the EBF1 3‟UTR that is capable of mediating the silencing of luciferase expression by BART11-5p but is not capable of silencing a full length EBF1-3‟UTR luciferase construct. This study provides evidence that EBF1 may be a target of one or more EBV-miRNAs.

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ID Code: 63702
Item Type: QUT Thesis (Masters by Research)
Supervisor: Gandhi, Maher & Nourse, Jamie
Keywords: BART11-5p, B cell differentiation, Bim, Early B cell factor (EBF1), Epstein-Barr virus (EBV), Methyl CpG binding protein 2 (MECP2), microRNA (miRNA), luciferase silencing assays
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Institution: Queensland University of Technology
Deposited On: 24 Oct 2013 06:40
Last Modified: 08 Sep 2015 23:47

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