The role of EBV-encoded microRNAs in B cell differentiation

Ross, Nathan Arlen (2012) The role of EBV-encoded microRNAs in B cell differentiation. Masters by Research thesis, Queensland University of Technology.

Abstract

Epstein Barr virus (EBV) is a common γ-herpes virus, infecting approximately 90% of the world‟s population. It is also one of the first known viruses known to be oncogenic, and is associated with a number of tumour types, primarily lymphomas.

MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression and many human miRNAs have been associated with the development of malignancies including cancer. EBV was the first human virus identified to express miRNAs and encodes more than 40 miRNAs within its genome. Yet, an understanding of the targets of EBV-miRNAs, and thereby the function of them in pathogenesis remains sadly limited.

This study identifies a potential novel target of EBV-miRNAs, MECP2 and characterises the miRNA:mRNA interactions between two previously identified novel targets; Bim and EBF1. In particular, this study focuses upon the interaction between EBF1 and the EBV-miRNA BART11-5p, demonstrating a 151bp region of the EBF1 3‟UTR that is capable of mediating the silencing of luciferase expression by BART11-5p but is not capable of silencing a full length EBF1-3‟UTR luciferase construct. This study provides evidence that EBF1 may be a target of one or more EBV-miRNAs.

Impact and interest:

Citation counts are sourced monthly from Scopus and Web of Science® citation databases.

These databases contain citations from different subsets of available publications and different time periods and thus the citation count from each is usually different. Some works are not in either database and no count is displayed. Scopus includes citations from articles published in 1996 onwards, and Web of Science® generally from 1980 onwards.

Citations counts from the Google Scholar™ indexing service can be viewed at the linked Google Scholar™ search.

Full-text downloads:

121 since deposited on 24 Oct 2013
43 in the past twelve months

Full-text downloads displays the total number of times this work’s files (e.g., a PDF) have been downloaded from QUT ePrints as well as the number of downloads in the previous 365 days. The count includes downloads for all files if a work has more than one.

ID Code: 63702
Item Type: QUT Thesis (Masters by Research)
Supervisor: Gandhi, Maher & Nourse, Jamie
Keywords: BART11-5p, B cell differentiation, Bim, Early B cell factor (EBF1), Epstein-Barr virus (EBV), Methyl CpG binding protein 2 (MECP2), microRNA (miRNA), luciferase silencing assays
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Institution: Queensland University of Technology
Deposited On: 24 Oct 2013 06:40
Last Modified: 08 Sep 2015 23:47

Export: EndNote | Dublin Core | BibTeX

Repository Staff Only: item control page