A rapid and sensitive UPLC-ESI MS method for analysis of isofraxidin, a natural antistress compound, and its metabolites in rat plasma

Sun, Hui, Lv, Haitao, Zhang, Yanmei, Wang, Xijun, Bi, Kaishun, & Cao, Hongxin (2007) A rapid and sensitive UPLC-ESI MS method for analysis of isofraxidin, a natural antistress compound, and its metabolites in rat plasma. Journal of Separation Science, 30(18), pp. 3202-3206.

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Isofraxidin is one of the main bioactive constituents in the root of Acanthopanax senticosus, which has antifatigue, antistress, and immuno-accommondating effects. In this study, an ultraperformance LC (UPLC)-ESI MS method was developed for analyzing isofraxidin and its metabolites in rat plasma. The analysis was performed on a UPLC coupled with ESI MS (quadropole MS tandem TOF MS). The lower LOD (LLOD) for isofraxidin was 0.25 ng/mL, the intraday precision was less than 10%, the interday precision was less than 10%, and the extraction recovery was more than 80%. Isofraxidin and two metabolites (M1 and M2) were detected in rat plasma after oral administration of isofraxidin, and the molecular polarities of M1 and M2 were both increased compared to isofraxidin. The metabolites were identified as 5,6-dihydroxyl-7-methoxycoumarin and 5-hydroxyl-6,7-dimethoxycoumarin when subjected to parent ion spectra, product ion spectra, and extract mass and element composition analyses.

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11 citations in Web of Science®

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ID Code: 64203
Item Type: Journal Article
Refereed: Yes
Keywords: Animals, Biological Agents/*blood/*chemistry, Chromatography, Liquid/*methods, Coumarins/*blood/*chemistry, Male, Molecular Structure, Rats, Rats, Wistar, Sensitivity and Specificity, Spectrometry, Mass, Electrospray Ionization/*methods, Time Factors
DOI: 10.1002/jssc.200700251
ISSN: 1615-9306
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 10 Nov 2013 23:38
Last Modified: 13 Dec 2013 00:24

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