A novel characterisation model for microvasulature changes following closed soft tissue trauma using micro-CT imaging

Barani Lonbani, Zohreh, Parker, Tony, Schuetz, Michael, & Steck, Roland (2013) A novel characterisation model for microvasulature changes following closed soft tissue trauma using micro-CT imaging. In Translational Research Institute (TRI) Inaugural Annual Poster Symposium, 19 July 2013, Princes Alexandra Hospital, Brisbane, Australia.

Abstract

INTRODUCTION

There is evidence that the reduction of blood perfusion caused by closed soft tissue trauma (CSTT) delays the healing of the affected soft tissues and bone [1]. We hypothesise that the characterisation of vascular morphology changes (VMC) following injury allows us to determine the effect of the injury on tissue perfusion and thereby the severity of the injury. This research therefore aims to assess the VMC following CSTT in a rat model using contrast-enhanced micro-CT imaging.

METHODOLOGY

A reproducible CSTT was created on the left leg of anaesthetized rats (male, 12 weeks) with an impact device. After euthanizing the animals at 6 and 24 hours following trauma, the vasculature was perfused with a contrast agent (Microfil, Flowtech, USA). Both hind-limbs were dissected and imaged using micro-CT for qualitative comparison of the vascular morphology and quantification of the total vascular volume (VV). In addition, biopsy samples were taken from the CSTT region and scanned to compare morphological parameters of the vasculature between the injured and control limbs.

RESULTS AND DISCUSSION

While the visual observation of the hindlimb scans showed consistent perfusion of the microvasculature with microfil, enabling the identification of all major blood vessels, no clear differences in the vascular architecture were observed between injured and control limbs. However, overall VV within the region of interest (ROI)was  measured to be higher for the injured limbs after 24h. Also, scans of biopsy samples demonstrated that vessel diameter and density were higher in the injured legs 24h after impact.

CONCLUSION

We believe these results will contribute to the development of objective diagnostic methods for CSTT based on changes to the microvascular morphology as well as aiding in the validation of future non-invasive clinical assessment modalities.

Impact and interest:

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ID Code: 64208
Item Type: Conference Item (Poster)
Refereed: Yes
Keywords: Micro-CT imaging, Closed soft tissue trauma, Microvasulature changes, Characterisation model
Divisions: Current > Schools > School of Biomedical Sciences
Current > Schools > School of Chemistry, Physics & Mechanical Engineering
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Copyright Owner: Copyright 2013 The authors and QUT
Copyright Statement: All rights reserved for authors and Queensland University of Technology
Deposited On: 11 Nov 2013 02:41
Last Modified: 11 Nov 2013 02:41

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