Quantifying alterations in cell migration : tracking fluorescently-tagged migrating cells by FACs and live-imaging

Murray, Rachael (2013) Quantifying alterations in cell migration : tracking fluorescently-tagged migrating cells by FACs and live-imaging. In Methods in Molecular Biology : Wound Regeneration and Repair. Humana Press/Springer, pp. 477-494.

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Abstract

Cell migration is fundamental to many different physiological processes including embryonic development, inflammation and wound healing. Given the range and importance cell migration plays a number of assays have been developed to measure different aspects of cell migration. Here we describe two different methods to analyze cell migration. The first method analyzes the migration of fluorescently tagged cells using Boyden chambers and FACs and the second looks at migration properties using time-lapse microscopy.

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ID Code: 64832
Item Type: Book Chapter
DOI: 10.1007/978-1-62703-505-7_28
ISBN: 9781627035040
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100)
Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000) > BIOCHEMISTRY AND CELL BIOLOGY (060100) > Cellular Interactions (incl. Adhesion Matrix Cell Wall) (060106)
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Deposited On: 27 Nov 2013 22:29
Last Modified: 24 Oct 2015 16:09

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