Identification of GABA receptors in chick retinal pigment epithelium

Cheng, Zhen-Ying, Wang, Xu-Ping, Schmid, Katrina L., & Liu, Lei (2013) Identification of GABA receptors in chick retinal pigment epithelium. Neuroscience Letters, 539, pp. 43-47.

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Purpose: The retinal pigment epithelium (RPE) is a multifunctional, monolayer of cells located between the neural retina and the choroicapillaris. γ-Aminobutyric acid (GABA) is the most important inhibitory neurotransmitter in the retina and GABA receptors are known to be present in chick retina, sclera and cornea. There is a report of genes involved in GABA receptor signaling being expressed in human RPE, however, whether GABA receptors are present in chick RPE is unknown.

Methods: Real time PCR and western blot were used to determine the expression of GABA receptors (alpha1 GABAA, GABABR2, and rho1 GABAC receptors) in isolated chicken RPE. Immunofluorescence using antibodies against one of the GABA receptor sub-types was used to determine receptor localization.

Results: Both real-time PCR and western blot demonstrated that alpha1 GABAA, GABABR2 and rho1 GABAC receptors were expressed in isolated chick RPE. Immunofluorescence further demonstrated that GABA receptors were localized to the cell membrane and plasma of RPE cells.

Conclusions: Alpha1 GABAA, GABABR2 and rho1 GABAC receptors were expressed in chick RPE. The purpose of the GABA receptors within the RPE remains to be explored.

Impact and interest:

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10 citations in Web of Science®

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ID Code: 64982
Item Type: Journal Article
Refereed: Yes
Keywords: Receptors, GABA, Chicken, Retinal pigment epithelium, Ocular physiology
DOI: 10.1016/j.neulet.2013.01.038
ISSN: 03043940
Subjects: Australian and New Zealand Standard Research Classification > MEDICAL AND HEALTH SCIENCES (110000) > OPTOMETRY AND OPHTHALMOLOGY (111300)
Divisions: Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Current > Schools > School of Optometry & Vision Science
Copyright Owner: Copyright 2013 Elsevier Ireland Ltd.
Deposited On: 03 Dec 2013 01:44
Last Modified: 09 Apr 2014 12:22

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