Matrix metalloproteinases 2 and 9 (gelatinases A and B) expression in malignant mesothelioma and benign pleura

Edwards, J. G., McLaren, J., Jones, J. L., Waller, D. A., & O'Byrne, Kenneth J. (2003) Matrix metalloproteinases 2 and 9 (gelatinases A and B) expression in malignant mesothelioma and benign pleura. British Journal of Cancer : BJC, 88(10), pp. 1553-1559.

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Abstract

Matrix metalloproteinases (MMPs), in particular the gelatinases (MMP-2 and -9), play a significant role in tumour invasion and angiogenesis. The expression and activities of MMPs have not been characterised in malignant mesothelioma (MM) tumour samples. In a prospective study, gelatinase activity was evaluated in homogenised supernatants of snap frozen MM (n = 35), inflamed pleura (IP, n = 12) and uninflammed pleura (UP, n = 14) tissue specimens by semiquantitative gelatin zymography. Matrix metalloproteinases were correlated with clinicopathological factors and with survival using Kaplan-Meier and Cox proportional hazard models. In MM, pro- and active MMP-2 levels were significantly greater than for MMP-9 (P = 0.006, P<0.001). Active MMP-2 was significantly greater in MM than in UP (P=0.04). MMP-2 activity was equivalent between IP and MM, but both pro- and active MMP-9 activities were greater in IP (P=0.02, P=0.009). While there were trends towards poor survival with increasing total and pro-MMP-2 activity (P=0.08) in univariate analysis, they were both independent poor prognostic factors in multivariate analysis in conjunction with weight loss (pro-MMP-2 P = 0.03, total MMP-2 P = 0.04). Total and pro-MMP-2 also contributed to the Cancer and Leukemia Group B prognostic groups. MMP-9 activities were not prognostic. Matrix metalloproteinases, and in particular MMP-2, the most abundant gelatinase, may play an important role in MM tumour growth and metastasis. Agents that reduce MMP synthesis and/or activity may have a role to play in the management of MM. © 2003 Cancer Research UK.

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ID Code: 65046
Item Type: Journal Article
Refereed: Yes
Additional URLs:
Keywords: Malignant mesothelioma, Matrix metalloproteinases, Prognosis, gelatinase A, gelatinase B, angiogenesis, article, benign tumor, cancer invasion, cancer survival, controlled study, human, human tissue, Kaplan Meier method, metastasis, multivariate analysis, pleura tumor, pleurisy, priority journal, protein expression, tumor growth, zymography, Adult, Aged, Disease Progression, Gene Expression Regulation, Neoplastic, Humans, Immunohistochemistry, Matrix Metalloproteinase 2, Matrix Metalloproteinase 9, Mesothelioma, Middle Aged, Neoplasm Metastasis, Prospective Studies, Survival
DOI: 10.1038/sj.bjc.6600920
ISSN: 0007-0920
Divisions: Current > Schools > School of Biomedical Sciences
Current > QUT Faculties and Divisions > Faculty of Health
Current > Institutes > Institute of Health and Biomedical Innovation
Copyright Owner: Copyright 2003 Nature Publishing Group
Deposited On: 03 Dec 2013 06:42
Last Modified: 03 Dec 2013 06:42

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