Evaluation of canine bone marrow-derived mesenchymal stem cells after long-term cryopreservation

Zhu, Xiaofeng, Yuan, Fang, Li, Houxuan, Zheng, Yuqian, Xiao, Yin, & Yan, Fuhua (2013) Evaluation of canine bone marrow-derived mesenchymal stem cells after long-term cryopreservation. Zoological Science, 30(12), pp. 1032-1037.

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Autologous bone marrow-derived mesenchymal stem cell (BMSCs)-based therapies show great potential in regenerative medicine. However, long-term storage and preservation of BMSCs for clinical use is still a great clinical challenge. The present study aimed to analyze the effect of long-term cryopreservation on the regenerative ability of BMSCs. After cryopreservation of BMSCs from beagle dogs for three years, cell viability, and quantitative analysis of alkaline phosphatase (ALP) activity, surface adherence, and mineralized nodule formation were analyzed. BMSCs in cell-scaffold complex were then implanted into nude mice. There was no significant difference in cell viability and ALP activity between osteogenic differentiation and non-osteogenic differentiation of BMSCs, and BMSCs in cell-scaffold complex retained osteogenic differentiation ability in vivo. These results indicate that long-term cryopreserved BMSCs maintain their have capacity to contribute to regeneration.

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ID Code: 65458
Item Type: Journal Article
Refereed: Yes
Keywords: bone marrow mesenchymal stem cell, cryopreservation, alkaline phosphatase, cell proliferation, differentiation
DOI: 10.2108/zsj.30.1032
ISSN: 0289-0003
Subjects: Australian and New Zealand Standard Research Classification > BIOLOGICAL SCIENCES (060000)
Divisions: Current > Institutes > Institute of Health and Biomedical Innovation
Current > QUT Faculties and Divisions > Science & Engineering Faculty
Deposited On: 19 Dec 2013 00:26
Last Modified: 02 Apr 2014 21:34

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